| Author | Chmelová, L'ubomíra | |
| Author | Kraeva, Natalya | |
| Author | Saura, Andreu | |
| Author | Krayzel, Adam | |
| Author | Vieira, Cecilia Stahl | |
| Author | Ferreira, Tainá Neves | |
| Author | Soares, Rodrigo Pedro | |
| Author | Bučková, Barbora | |
| Author | Galan, Arnau | |
| Author | Horáková, Eva | |
| Author | Vojtková, Barbora | |
| Author | Sádlová, Jovana | |
| Author | Malysheva, Marina N. | |
| Author | Butenko, Anzhelika | |
| Author | Prokopchuk, Galina | |
| Author | Frolov, Alexander O. | |
| Author | Lukeš, Julius | |
| Author | Horváth, Anton | |
| Author | Škodová-Sveráková, Ingrid | |
| Author | Feder, Denise | |
| Author | Kostygov, Alexei Yu | |
| Author | Yurchenko, Vyacheslav | |
| Access date | 2024-06-27T14:17:52Z | |
| Available date | 2024-06-27T14:17:52Z | |
| Document date | 2024 | |
| Citation | CHMELOVÁ, L'ubomíra et al. Intricate balance of dually-localized catalase modulates infectivity of Leptomonas seymouri (Kinetoplastea: Trypanosomatidae). International Journal for Parasitology, v. 54, n. 8-9, p. 391-400, Jul. 2024. | en_US |
| ISSN | 0020-7519 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/64706 | |
| Language | eng | en_US |
| Publisher | Pergamon Press | en_US |
| Rights | restricted access | |
| Title | Intricate balance of dually-localized catalase modulates infectivity of Leptomonas seymouri (Kinetoplastea: Trypanosomatidae) | en_US |
| Type | Article | |
| DOI | 10.1016/j.ijpara.2024.04.007 | |
| Abstract | Nearly all aerobic organisms are equipped with catalases, powerful enzymes scavenging hydrogen peroxide and facilitating defense against harmful reactive oxygen species. In trypanosomatids, this enzyme was not present in the common ancestor, yet it had been independently acquired by different lineages of monoxenous trypanosomatids from different bacteria at least three times. This observation posited an obvious question: why was catalase so "sought after" if many trypanosomatid groups do just fine without it? In this work, we analyzed subcellular localization and function of catalase in Leptomonas seymouri. We demonstrated that this enzyme is present in the cytoplasm and a subset of glycosomes, and that its cytoplasmic retention is H2O2-dependent. The ablation of catalase in this parasite is not detrimental in vivo, while its overexpression resulted in a substantially higher parasite load in the experimental infection of Dysdercus peruvianus. We propose that the capacity of studied flagellates to modulate the catalase activity in the midgut of its insect host facilitates their development and protects them from oxidative damage at elevated temperatures. | en_US |
| Affilliation | Life Science Research Centre. Faculty of Science. University of Ostrava. Ostrava, Czechia. | en_US |
| Affilliation | Life Science Research Centre. Faculty of Science. University of Ostrava. Ostrava, Czechia. | en_US |
| Affilliation | Life Science Research Centre. Faculty of Science. University of Ostrava. Ostrava, Czechia. | en_US |
| Affilliation | Life Science Research Centre. Faculty of Science. University of Ostrava. Ostrava, Czechia. | en_US |
| Affilliation | Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia. Niterói, RJ, Brazil. | en_US |
| Affilliation | Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia. Niterói, RJ, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Biotecnologia Aplicada a Patógenos. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Biochemistry. Faculty of Natural Sciences. Comenius University. Bratislava, Slovakia. | en_US |
| Affilliation | Life Science Research Centre. Faculty of Science. University of Ostrava. Ostrava, Czechia. | en_US |
| Affilliation | Institute of Parasitology. Biology Centre. Czech Academy of Sciences. České Budějovice, Czechia. | en_US |
| Affilliation | Department of Parasitology. Faculty of Science. Charles University. Prague, Czechia. | en_US |
| Affilliation | Department of Parasitology. Faculty of Science. Charles University. Prague, Czechia. | en_US |
| Affilliation | Zoological Institute. Russian Academy of Sciences. St. Petersburg, Russia. | en_US |
| Affilliation | Life Science Research Centre. Faculty of Science. University of Ostrava. Ostrava, Czechia / Institute of Parasitology. Biology Centre, Czech Academy of Sciences. České Budějovice. Czechia / Faculty of Science. University of South Bohemia. České Budějovice, Czechia. | en_US |
| Affilliation | Institute of Parasitology. Biology Centre. Czech Academy of Sciences. České Budějovice, Czechia / Faculty of Science. University of South Bohemia. České Budějovice, Czechia. | en_US |
| Affilliation | Zoological Institute. Russian Academy of Sciences. St. Petersburg, Russia. | en_US |
| Affilliation | Institute of Parasitology. Biology Centre. Czech Academy of Sciences. České Budějovice, Czechia / Faculty of Science. University of South Bohemia. České Budějovice, Czechia. | en_US |
| Affilliation | Department of Biochemistry. Faculty of Natural Sciences. Comenius University. Bratislava, Slovakia. | en_US |
| Affilliation | Life Science Research Centre. Faculty of Science. University of Ostrava. Ostrava, Czechia / Department of Biochemistry. Faculty of Natural Sciences. Comenius University. Bratislava, Slovakia / Institute of Parasitology. Biology Centre. Czech Academy of Sciences. České Budějovice, Czechia. | en_US |
| Affilliation | Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia. Niterói, RJ, Brazil / Universidade Federal Fluminense. Instituto de Biologia. Laboratório de Biologia de Insetos. Niterói, RJ, Brazil / Instituto Nacional de Entomologia Molecular. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Life Science Research Centre. Faculty of Science. University of Ostrava. Ostrava, Czechia / Zoological Institute. Russian Academy of Sciences. St. Petersburg, Russia. | en_US |
| Affilliation | Life Science Research Centre. Faculty of Science. University of Ostrava. Ostrava, Czechia. | en_US |
| Subject | Catalase | en_US |
| Subject | Hydrogen peroxide | en_US |
| Subject | Trypanosomatids | en_US |
| Embargo date | 2030-12-31 | |
