| Author | Oliveira, B. M. D. S. | |
| Author | Costa, M. N. B. | |
| Author | Fontes, J. L. M. | |
| Author | Mengel, J. | |
| Author | Cardillo, F. | |
| Access date | 2024-07-04T19:05:19Z | |
| Available date | 2024-07-04T19:05:19Z | |
| Document date | 2023 | |
| Citation | OLIVEIRA, B. M. D. S. et al. Use of adjuvant therapies and their antitumor effects: bacillus calmette-guérin and the effect of splenectomy on the regulation of B16F0 melanoma in C57BL/6. In: CONGRESS OF THE BRAZILIAN SOCIETY OF IMMUNOLOGY, 46, 2023, Ouro Preto, MG. Anais eletrônicos [...] Ouro Preto, MG: SBI, 02 a 6 de outubro de 2023. p.1-33. | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/64846 | |
| Language | eng | en_US |
| Publisher | Sociedade Brasileira de Imunologia | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Melanoma | en_US |
| Subject in Portuguese | Imunregulação | en_US |
| Subject in Portuguese | BCG | en_US |
| Title | Use of adjuvant therapies and their antitumor effects: Bacillus Calmette-Guérin and the effect of splenectomy on the regulation of B16F0 melanoma in C57BL/6 | en_US |
| Type | Papers presented at events | en_US |
| Abstract | INTRODUCTION: Melanoma, a highly metastatic tumor, is known for its propensity to spread. Studies indicate that the spleen plays a role in creating a tumor microenvironment enriched with factors that promote tumor growth and regulatory cells. Consequently, investigating the impact of splenectomy and combining it with adjuvant treatment using Bacillus Calmette Guérin (BCG) becomes intriguing. OBJECTIVE: This project primarily aims to examine cells with regulatory potential and comprehend the composition and functional activity of infiltrated cells within the tumor following splenectomy or BCG vaccination. METHODS: C57BL/6 mice will be injected with 5x104 B16F0 tumor cells directly into the pinna of the ear. BCG, consisting of 1x107 organisms, will be administered locally or systemically ten days later. Some mice will undergo splenectomy, while others will receive BCG vaccination. Histological analysis will be conducted on tumor-containing sections of the ear, stained with HE for qualitative and quantitative evaluation. RESULTS: Animals with BCG-treated tumors (B16F0+BCG) exhibited increased infiltration of inflammatory cells, reduced necrosis, diminished desmoplastic reaction, and fewer tumor cells infiltrating the muscle. Furthermore, they demonstrated improved survival and better containment of the tumor at the injection site. Preliminary findings also suggest enhanced survival in animals that underwent splenectomy, with smaller tumor sizes observed in this group. Additionally, we observed an increase in spleen size as the tumor established itself. CONCLUSIONS: BCG treatment or splenectomy resulted in increased survival rates. In experimental melanoma, BCG vaccination improved inflammation, reducing necrosis, desmoplastic reaction, and muscular tumor infiltration. Our laboratory is currently conducting flow cytometry analysis on T lymphocytes infiltrating the tumor cells in the ear's pinna. Supported by: CAPES, CNPq and FIOCRUZ. | en_US |
| Affilliation | Universidade Federal da Bahia. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil. | en_US |
| Subject | Melanoma | en_US |
| Subject | Immunoregulation | en_US |
| Subject | BCG | en_US |
| DeCS | Melanoma | en_US |
| DeCS | Mycobacterium bovis | en_US |
