| Author | Barbosa, Lúcio Macedo | |
| Author | Silva, L. M. | |
| Author | Coelho, Paulo Marcos Zach | |
| Author | Santos, S. R. | |
| Author | Fortes Dias, Consuelo Latorre | |
| Access date | 2013-05-13T14:42:30Z | |
| Available date | 2013-05-13T14:42:30Z | |
| Document date | 2006 | |
| Citation | BARBOSA, L; et al. Primary culture of the region of the amebocyte-producing organ of the snail Biomphalaria glabrata, the intermediate host of Schistosoma mansoni. Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 101(6): 639-643, 2006 | pt_BR |
| ISSN | 0074-0276 | |
| URI | https://www.arca.fiocruz.br/handle/icict/6486 | |
| Sponsorship | Fundação Ezequiel Dias, Centro de Pesquisas
René-Rachou, CNPq | pt_BR |
| Language | eng | pt_BR |
| Rights | open access | pt_BR |
| Title | Primary culture of the region of the amebocyte-producing organ of the snail Biomphalaria glabrata, the intermediate host of Schistosoma mansoni | pt_BR |
| Type | Article | pt_BR |
| Abstract | Biomphalaria glabrata snails are major hosts for the digenetic trematoda Schistosoma mansoni, the causative agent of human schistosomiasis. The success or failure of the infection will be dependent on the mobilization of the molluskan internal defense system, where a major role will be played by circulating hemocytes produced by the APO (amebocyte-producing organ) of the snail. In this report, the primary culture of the APO region of B. glabrata was obtained for the first time, as well as a control culture of the ovotestis. Three different cell populations migrated easily from the explants in culture, with no need of any dispersion agent. The cells grew in suspension at anincubation temperature of 15ºC and the cultures were maintained viable for up to two weeks. Two of these cell populations obtained resembled cell types known to be present in the hemolymph of Biomphalaria. The availabilityof APO cells in culture may contribute to a better understanding of the internal defense in mollusks, in general, as well as the specific response of B. glabrata to S. mansoni infection. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Laboratório de Biologia Molecular e Celular. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil/Santa Casa de Misericórdia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Laboratório de Biologia Molecular e Celular. Belo Horizonte, MG, Brasil /Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Laboratório de Biologia Molecular e Celular. Belo Horizonte, MG, Brasil | pt_BR |
| Subject | Biomphalaria | pt_BR |
| Subject | schistosomiasis | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | hemocyte | pt_BR |
| Subject | tissue culture | pt_BR |
