| Author | Matos, Ada da Silva | |
| Author | Soares, Isabela Ferreira | |
| Author | Silva, Rodrigo Nunes Rodrigues da | |
| Author | Rodolphi, Cinthia Magalhães | |
| Author | Albrecht, Letusa | |
| Author | Donassolo, Rafael Amaral | |
| Author | Camacho, Cesar Lopez | |
| Author | Ano Bom, Ana Paula Dinis | |
| Author | Neves, Patrícia Cristina da Costa | |
| Author | Conte, Fernando de Paiva | |
| Author | Riccio, Lilian Rose Pratt | |
| Author | Ribeiro, Cláudio Tadeu Daniel | |
| Author | Totino, Paulo Renato Rivas | |
| Author | Lima Junior, Josué da Costa | |
| Access date | 2024-07-08T13:26:59Z | |
| Available date | 2024-07-08T13:26:59Z | |
| Document date | 2024 | |
| Citation | MATOS, Ada da Silva et al. Immunogenicity of PvCyRPA, PvCelTOS and Pvs25 chimeric recombinant protein of Plasmodium vivax in murine model. Frontiers in Immunology, v. 15, p. 1-15, 19 June 2024. | en_US |
| ISSN | 1664-3224 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/64873 | |
| Description | Produção científica do Laboratório de Hantaviroses e Rickettsioses. | pt_BR |
| Description | Produção científica do Laboratório de Imunoparasitologia. | pt_BR |
| Description | Produção científica do Laboratório de Pesquisa em Malária. | pt_BR |
| Sponsorship | The author(s) declare financial support was received for the research, authorship, and/or publication of this article. | |
| Sponsorship | This study was supported by the Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) (E26/202.854/2019-JCNE to Josué da Costa Lima Junior and E-26/202.921/2018 to Cláudio Tadeu Daniel Ribeiro; and E26/211.112/2019-Apoio a Grupos Emergentes de Pesquisa), and the Inovabio grant program, FIOCRUZ (PAEF II, IOC-008-FIO-22-2-49). | |
| Sponsorship | Cláudio Tadeu Daniel Ribeiro and Josué da Costa Lima Junior received a Productivity Research Fellowship from CNPq. | |
| Sponsorship | The APC was funded by Fiocruz. | |
| Sponsorship | The Malaria Research Laboratory, IOC, Fiocruz is an Associated Laboratory of the Instituto Nacional de Ciência e Tecnologia (INCT) in Neuroimmunomodulation and of the Neuro-inflammation Network of Faperj. | |
| Language | eng | en_US |
| Publisher | Frontiers Media | en_US |
| Rights | open access | en_US |
| Title | Immunogenicity of PvCyRPA, PvCelTOS and Pvs25 chimeric recombinant protein of Plasmodium vivax in murine model | en_US |
| Type | Article | en_US |
| DOI | 10.3389/fimmu.2024.1392043 | |
| Abstract | In the Americas, P. vivax is the predominant causative species of malaria, a debilitating and economically significant disease. Due to the complexity of the malaria parasite life cycle, a vaccine formulation with multiple antigens expressed in various parasite stages may represent an effective approach. Based on this, we previously designed and constructed a chimeric recombinant protein, PvRMC-1, composed by PvCyRPA, PvCelTOS, and Pvs25 epitopes. This chimeric protein was strongly recognized by naturally acquired antibodies from exposed population in the Brazilian Amazon. However, there was no investigation about the induced immune response of PvRMC-1. Therefore, in this work, we evaluated the immunogenicity of this chimeric antigen formulated in three distinct adjuvants: Stimune, AddaVax or Aluminum hydroxide (Al(OH)3) in BALB/c mice. Our results suggested that the chimeric protein PvRMC-1 were capable to generate humoral and cellular responses across all three formulations. Antibodies recognized full-length PvRMC-1 and linear B-cell epitopes from PvCyRPA, PvCelTOS, and Pvs25 individually. Moreover, mice’s splenocytes were activated, producing IFN-γ in response to PvCelTOS and PvCyRPA peptide epitopes, affirming T-cell epitopes in the antigen. While aluminum hydroxide showed notable cellular response, Stimune and Addavax induced a more comprehensive immune response, encompassing both cellular and humoral components. Thus, our findings indicate that PvRMC-1 would be a promising multistage vaccine candidate that could advance to further preclinical studies. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Parasitologia, Microbiologia e Imunologia. Setor de Parasitologia. Núcleo de Pesquisa em Parasitologia. Juiz de Fora, MG, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Pesquisa em Apicomplexa. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Pesquisa em Apicomplexa. Curitiba, PR, Brasil. | en_US |
| Affilliation | University of Oxford. The Jenner Institute. Nuffield Department of Medicine. Oxford, United Kingdom. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Imunológica. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Imunológica. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório Piloto de Eucariontes. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Escola Politécnica de Saúde Joaquim Venâncio. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Plasmodium vivax | en_US |
| Subject | Multistage chimeric protein | en_US |
| Subject | Immunization | en_US |
| Subject | BALB/c | en_US |
| Subject | In silico simulation | en_US |
| Subject | Vaccine | en_US |
| Subject | Immunogenicity | en_US |
| e-ISSN | 1664-3224 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
