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AutorDomingues, Cinthya Fonseca
AutorCastro, Tatiana Xavier de
AutorLago, Bárbara Vieira do
AutorGarcia, Rita de Cássia Nasser Cubel
Fecha de acceso2024-07-11T12:57:37Z
Fecha de disponibilización2024-07-11T12:57:37Z
Fecha de publicación2024
ReferenciaDOMINGUES, Cinthya Fonseca et al. Genetic characterization of the parvovirus full-length VP2 gene in domestic cats in Brazil. Research in Veterinary Science, v. 170, p. 1-8, Apr. 2024.
ISSN0034-5288
URIhttps://www.arca.fiocruz.br/handle/icict/64924
DescripciónProdução científica do Laboratório de Hepatites Virais.pt_BR
PromociónThis study was supported by Fundação de Amparo à Pesquisa Carlos Chagas Filho - FAPERJ (Grant No E-26/110.990/2013). Cinthya Fonseca Domingues was the Master student recipient of a scholarship from "Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)" for participating in the Graduate Program of the "Microbiologia e Parasitologia Aplicadas," Universidade Federal Fluminense.en_US
Idiomaengen_US
EditorElsevier
Derechos de autorrestricted access
TítuloGenetic characterization of the parvovirus full-length VP2 gene in domestic cats in Brazilen_US
Tipo del documentoArticle
DOI10.1016/j.rvsc.2024.105186
Resumen en InglésFeline parvovirus (FPV) and canine parvovirus (CPV) are over 98% identical in their DNA sequences, and the new variants of CPV (2a/2b/2c) have gained the ability to infect and replicate in cats. The aim of this study was to determine the genetic diversity in the VP2 gene of parvovirus strains circulating in domestic cats in Brazil during a 10-year period (2008–2017). For parvovirus screening, specific PCR was performed, and 25 (34.7%) of 72 cats tested positive. The PCR-positive samples were further subjected to full-length VP2 sequencing (1755 bp), and eight sequences (36%) were characterized as FPV, seven (28%) as CPV-2a and (32%) nine (36%) as CPV-2b. One sequence (RJ1085/11) showing typical CPV amino acid (aa) at residues 80 R, 93 N, 103 A, 232 I, and 323 N could not be characterized at this time. The sequences in this study displayed aa changes previously described for FPV (A14T, A91S, I101T, N564S, and A568G) from cats and CPV-2a/2b (S297N and Y324L) from dogs. However, the Y324L mutation has not yet been reported in any CPV-2a/2b strains from cats. Phylogenetic analysis supported the division of these sequences into two well-defined clades, clade 1 for FPV and clade 2 for CPV2a/2b. Unusually, the sequence RJ1085/11 was grouped separately. Two recombination breakpoints were detected by Bootscan and 3Seq methods implemented in the RDP4. This study is the first report of CPV-2a/2b in cats in Brazil. The detection of FPV strains with mutations characteristic of CPV indicates that Brazilian FPV strains have undergone genetic changes.en_US
AfiliaciónUniversidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.
AfiliaciónUniversidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
AfiliaciónUniversidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.
Palavras clave en InglêsFeline parvovirusen_US
Palavras clave en InglêsCanine parvovirusen_US
Palavras clave en InglêsBrazilen_US
Palavras clave en InglêsRecombinationen_US
Palavras clave en InglêsGenetic analysisen_US
e-ISSN1532-2661
xmlui.metadata.dc.subject.ods03 Saúde e Bem-Estar
xmlui.metadata.dc.subject.ods15 Vida terrestre


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