| Author | Seydel, Clara Monteiro | |
| Author | Gonzaga, Beatriz Matheus de Souza | |
| Author | Coelho, Laura Lacerda | |
| Author | Garzoni, Luciana Lopes de Almeida Ribeiro | |
| Access date | 2024-07-30T12:34:48Z | |
| Available date | 2024-07-30T12:34:48Z | |
| Document date | 2024 | |
| Citation | SEYDEL, Clara Monteiro et al. Exploring the dimensions of pre-clinical research: 3D cultures as an investigative model of cardiac fibrosis in Chagas disease. Biomedicines, v. 12, n. 7, p. 1-11, 25 June 2024. | |
| ISSN | 2227-9059 | |
| URI | https://www.arca.fiocruz.br/handle/icict/65207 | |
| Description | Produção científica do Laboratório de Inovações em Terapias, Ensino e Bioprodutos. | pt_BR |
| Sponsorship | The author(s) declare financial support was received for the research, authorship, and/or publication of this article. | |
| Sponsorship | The authors would like to thank Oswaldo Cruz Institute, FAPERJ, CAPES and CNPq. | |
| Sponsorship | This project has also been supported by Brazilian National Congress Parliamentary amendments No. 13380024 and 13380014. | |
| Language | eng | en_US |
| Publisher | MDPI | |
| Rights | open access | |
| Title | Exploring the dimensions of pre-clinical research: 3D cultures as an investigative model of cardiac fibrosis in Chagas disease | en_US |
| Type | Article | |
| DOI | 10.3390/biomedicines12071410 | |
| Abstract | A three-dimensional (3D) cell culture can more precisely mimic tissues architecture and functionality, being a promising alternative model to study disease pathophysiology and drug screening. Chagas disease (CD) is a neglected parasitosis that affects 7 million people worldwide. Trypanosoma cruzi’s (T. cruzi) mechanisms of invasion/persistence continue to be elucidated. Benznidazole (BZ) and Nifurtimox (NF) are trypanocidal drugs with few effects on the clinical manifestations of the chronic disease. Chronic Chagas cardiomyopathy (CCC) is the main manifestation of CD due to its frequency and severity. The development of fibrosis and hypertrophy in cardiac tissue can lead to heart failure and sudden death. Thus, there is an urgent need for novel therapeutic options. Our group has more than fifteen years of expertise using 3D primary cardiac cell cultures, being the first to reproduce fibrosis and hypertrophy induced by T. cruzi infection in vitro. These primary cardiac spheroids exhibit morphological and functional characteristics that are similar to heart tissue, making them an interesting model for studying CD cardiac fibrosis. Here, we aim to demonstrate that our primary cardiac spheroids are great preclinical models which can be used to develop new insights into CD cardiac fibrosis, presenting advances already achieved in the field, including disease modeling and drug screening. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil. | |
| Subject | Three-dimensional cell culture 1 | en_US |
| Subject | Cardiac spheroids 2 | en_US |
| Subject | Chagas disease 3 | en_US |
| Subject | Therapy 4 | en_US |
| e-ISSN | 2227-9059 | |
