| Author | Montilla-Suárez, Jerica M. | |
| Author | Santos, Aline Loures dos | |
| Author | Araújo, Bianca de | |
| Author | Rodrigues, Júlia H. V. | |
| Author | Tenorio, Juan C. | |
| Author | Soares, Milena B. P. | |
| Author | Silva, Valdenizia R. | |
| Author | Santos, Luciano de S. | |
| Author | Bezerra, Daniel P. | |
| Author | Taylor, Jason G. | |
| Author | Correa, Rodrigo S. | |
| Access date | 2024-08-12T19:55:09Z | |
| Available date | 2024-08-12T19:55:09Z | |
| Document date | 2024 | |
| Citation | MONTILLA-SUAREZ, Jerica M. et al. Structural elucidation of a new binuclear copper (II)/phenanthroline/ 2-(2,4-dihydroxybenzoyl) benzoate complex by synchrotron radiation, DNA interaction and cytotoxicity against tumor cells. Inorganic Chemistry Communications, v.167, p. 1-10, 2024. | en_US |
| ISSN | 1879-0259 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/65336 | |
| Sponsorship | Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG).
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Universidade Federal de Ouro Preto (UFOP). | en_US |
| Language | eng | en_US |
| Publisher | Elsevier | en_US |
| Rights | restricted access | |
| Subject in Portuguese | Binuclear | en_US |
| Subject in Portuguese | Estrutura cristalina | en_US |
| Subject in Portuguese | Sistema híbrido | en_US |
| Subject in Portuguese | Ensaio de citotoxicidade | en_US |
| Title | Structural elucidation of a new binuclear copper(II)/phenanthroline/ 2-(2,4-dihydroxybenzoyl)benzoate complex by synchrotron radiation, DNA interaction and cytotoxicity against tumor cells | en_US |
| Type | Article | |
| DOI | 10.1016/j.inoche.2024.112670 | |
| Abstract | A novel binuclear copper(II)-based complex was obtained with 2-(2,4-dihydroxybenzoyl)benzoate (DBB) and 1,10-phenanthroline (phen) as chelate ligands (Complex 1). Characterization was performed by ultraviolet, visible and infrared spectroscopy, and Synchrotron X-ray diffraction (SXRD), confirming the chemical structure. The crystal structure presents each metal center of complex 1 with a distorted square pyramidal geometry. Such as observed, one metal ion shows of one DBB and one phen as bidentate, as well as one monodentate methanol at the axial position. Meanwhile, the other copper center is also formed by one DBB and phen as bidentate ligands, and an additional carboxylate of DBB as monodentate, making a bridge between the two nuclei of the complex. Alamar blue assays revealed that complex 1 is cytotoxic against HCT116 (human colon carcinoma), and HepG2 (human hepatocellular carcinoma) tumor cell lines, with IC50 values of 5 and 2.75 μg.mL−1, respectively. HCT116 cells treated with complex 1 showed higher levels of activated caspase-3 and cleaved PARP (Asp214), as well as higher DNA fragmentation. | en_US |
| Affilliation | Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Departamento de Química. Ouro Preto, MG, Brasil. | en_US |
| Affilliation | Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Departamento de Química. Ouro Preto, MG, Brasil. | en_US |
| Affilliation | Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Departamento de Química. Ouro Preto, MG, Brasil. | en_US |
| Affilliation | Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Departamento de Química. Ouro Preto, MG, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Centro Universitário SENAI/CIMATEC. Instituto SENAI de Inovação (ISI) Em Sistemas Avançados Em Saúde. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Departamento de Química. Ouro Preto, MG, Brasil. | en_US |
| Affilliation | Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Departamento de Química. Ouro Preto, MG, Brasil. | en_US |
| Subject | Binuclear | en_US |
| Subject | Crystal structure | en_US |
| Subject | Hybrid system | en_US |
| Subject | Cytotoxicity assay | en_US |
| DeCS | Técnicas do sistema de duplo-híbrido | en_US |
| Embargo date | 2026 | |
