Author | Mattos, Ana Carolina Alves de | |
Author | Kusel, John Robert | |
Author | Pimenta, Paulo Filemon Paolucci | |
Author | Coelho, Paulo Marcos Zach | |
Access date | 2013-06-06T14:16:12Z | |
Available date | 2013-06-06T14:16:12Z | |
Document date | 2006 | |
Citation | MATTOS, Ana Carolina Alves de et al. Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, suppl.1, p. 283-287, 2006. | pt_BR |
ISSN | 0074-0276 | |
URI | https://www.arca.fiocruz.br/handle/icict/6570 | |
Sponsorship | Fiocruz-CNPq | pt_BR |
Language | eng | pt_BR |
Rights | open access | pt_BR |
Title | Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/S0074-02762006000900044 | |
Abstract | Praziquantel (PZQ) is effective against all the evolutive phases of Schistosoma mansoni. Infected Biomphalaria glabrata snails have their cercarial shedding interrupted when exposed to PZQ. Using primary in vitro transformed sporocysts, labeled with the probe Hoechst 33258 (indicator of membrane integrity), and lectin of Glycine max (specific for carbohydrate of N-acetylgalactosamine membrane), we evaluated the presence of lysosomes at this evolutive phase of S. mansoni, as well as the influence of PZQ on these acidic organelles and on the tegument of the sporocyst. Although the sporocyst remained alive, it was observed that there was a marked contraction of its musculature, and there occurred a change in the parasite’s structure. Also, the acidic vesicles found in the sporo-cysts showed a larger delimited area after contact of the parasites with PZQ. Damages to the tegument was also observed, as show a well-marked labeling either with Hoechst 33258 or with lectin of Glycine max after contact of sporocysts with the drug. These results could partially explain the interruption/reduction mechanism of cercarial shedding in snails exposed to PZQ. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | University of Glasgow. IBLS Division of Infection and Immunity. Scotland, UK. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil / Santa Casa de Misericórdia de Belo Horizonte. Belo Horizonte, MG, Brasil. | pt_BR |
Subject | Praziquantel | pt_BR |
Subject | Fluorescence | pt_BR |
Subject | Hoechst 33258 | pt_BR |
Subject | LysoTracker Red | pt_BR |
Subject | Lectin of Glycine max | pt_BR |
Subject | Sporocysts | pt_BR |
Subject | Schistosoma mansoni | pt_BR |