| Author | Fernandes, Gabriela M. | |
| Author | Rodrigues-Mattos, Guilherme H. | |
| Author | Torres, Letícia M. | |
| Author | Guedes, Karla S. | |
| Author | Fontes, Cor J. F. | |
| Author | Ntumngia, Francis B. | |
| Author | Adams, John H. | |
| Author | Brito, Cristiana Ferreira Alves de | |
| Author | Kano, Flora Satiko | |
| Author | Sousa, Taís Nobrega de | |
| Author | Carvalho, Luzia Helena | |
| Access date | 2024-09-16T19:52:33Z | |
| Available date | 2024-09-16T19:52:33Z | |
| Document date | 2024 | |
| Citation | FERNANDES, Gariela M. et al. Natural genetic diversity of the DBL domain of a novel member of the Plasmodium vivax erythrocyte binding-like proteins (EBP2) in the Amazon rainforest. Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, v. 123, p. 1-8, Sept. 2024. | en_US |
| ISSN | 1567-1348 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/65907 | |
| Language | eng | en_US |
| Publisher | Elsevier | en_US |
| Rights | restricted access | |
| Title | Natural genetic diversity of the DBL domain of a novel member of the Plasmodium vivax erythrocyte binding-like proteins (EBP2) in the Amazon rainforest | en_US |
| Type | Article | |
| DOI | 10.1016/j.meegid.2024.105628 | |
| Abstract | In malaria parasites, the erythrocyte binding-like proteins (EBL) are a family of invasion proteins that are attractive vaccine targets. In the case of Plasmodium vivax, the widespread malaria parasite, blood-stage vaccines have been largely focused on a single EBL candidate, the Duffy binding-like domain (DBL) of the Duffy binding protein (DBPII), due to its well-characterized role in the reticulocyte invasion. A novel P. vivax EBL family member, the Erythrocyte binding protein (EBP2, also named EBP or DBP2), binds preferentially to reticulocytes and may mediate an alternative P. vivax invasion pathway. To gain insight into the natural genetic diversity of the DBL domain of EBP2 (region II; EBP2-II), we analyzed ebp2-II gene sequences of 71 P. vivax isolates collected in different endemic settings of the Brazilian Amazon rainforest, where P. vivax is the predominant malaria-associated species. Although most of the substitutions in the ebp2-II gene were non-synonymous and suggested positive selection, the results showed that the DBL domain of the EBP2 was much less polymorphic than that of DBPII. The predominant EBP2 haplotype in the Amazon region corresponded to the C127 reference sequence first described in Cambodia (25% C127-like haplotype). An overview of ebp2-II gene sequences available at GenBank (n = 352) from seven countries (Cambodia, Madagascar, Myanmar, PNG, South Korea, Thailand, Vietnam) confirmed the C127-like haplotype as highly prevalent worldwide. Two out of 43 haplotypes (5 to 20 inferred per country) showed a global frequency of 60%. The results presented here open new avenues of research pursuit while suggesting that a vaccine based on the DBL domain of EBP2 should target a few haplotypes for broad coverage. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Faculdade Ciências Médicas de Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Mato Grosso. Faculdade de Medicina. Julio Muller School Hospital. Cuiabá, MT, Brazil. | en_US |
| Affilliation | Universidade Federal de Mato Grosso. Faculdade de Medicina. Julio Muller School Hospital. Cuiabá, MT, Brazil. | en_US |
| Affilliation | Center for Global Health and Infectious Diseases Research. College of Public Health. University of South Florida. Tampa, FL, USA. | en_US |
| Affilliation | Center for Global Health and Infectious Diseases Research. College of Public Health. University of South Florida. Tampa, FL, USA. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil / Department of Microbiology, Tumor and Cell Biology. Karolinska Institutet. Solna, Sweden. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brazil. | en_US |
| Subject | Erythrocyte binding protein 2 | en_US |
| Subject | Genetic polymorphism | en_US |
| Subject | Malaria | en_US |
| Subject | Plasmodium vivax | en_US |
| Subject | EBP2 gene | en_US |
| Embargo date | 2030-12-31 | |
