| Author | Moreno, Keldenn Melo Farias | |
| Author | Andrade, Virgínia Antunes de | |
| Author | Iani, Felipe Campos de Melo | |
| Author | Fonseca, Vagner | |
| Author | Lima, Maurício Teixeira | |
| Author | Barbosa, Emerson de Castro | |
| Author | Tomé, Luiz Marcelo Ribeiro | |
| Author | Guimarães, Natália Rocha | |
| Author | Fritsch, Hegger Machado | |
| Author | Adelino, Talita | |
| Author | Fereguetti, Tatiana Oliveira | |
| Author | Aspahan, Maíra Cardoso | |
| Author | Barros, Tereza Gamarano | |
| Author | Alcantara, Luiz Carlos Junior | |
| Author | Giovanetti, Marta | |
| Access date | 2024-09-17T16:53:29Z | |
| Available date | 2024-09-17T16:53:29Z | |
| Document date | 2024 | |
| Citation | MORENO, Keldenn Melo Farias et al. Exploring Microorganisms Associated to Acute Febrile Illness and Severe Neurological Disorders of Unknown Origin: A Nanopore Metagenomics Approach. Genes, v. 15, n. 7, 922, 2024. doi: 10.3390/genes15070922. | en_US |
| ISSN | 2073-4425 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/65914 | |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | en_US |
| Title | Exploring Microorganisms Associated to Acute Febrile Illness and Severe Neurological Disorders of Unknown Origin: A Nanopore Metagenomics Approach. | en_US |
| Type | Article | en_US |
| Abstract | Acute febrile illness (AFI) and severe neurological disorders (SNDs) often present diagnostic challenges due to their potential origins from a wide range of infectious agents. Nanopore metagenomics is emerging as a powerful tool for identifying the microorganisms potentially responsible for these undiagnosed clinical cases. In this study, we aim to shed light on the etiological agents underlying AFI and SND cases that conventional diagnostic methods have not been able to fully elucidate. Our approach involved analyzing samples from fourteen hospitalized patients using a comprehensive nanopore metagenomic approach. This process included RNA extraction and enrichment using the SMART-9N protocol, followed by nanopore sequencing. Subsequent steps involved quality control, host DNA/cDNA removal, de novo genome assembly, and taxonomic classification. Our findings in AFI cases revealed a spectrum of disease-associated microbes, including Escherichia coli, Streptococcus sp., Human Immunodeficiency Virus 1 (Subtype B), and Human Pegivirus. Similarly, SND cases revealed the presence of pathogens such as Escherichia coli, Clostridium sp., and Dengue virus type 2 (Genotype-II lineage). This study employed a metagenomic analysis method, demonstrating its efficiency and adaptability in pathogen identification. Our investigation successfully identified pathogens likely associated with AFI and SNDs, underscoring the feasibility of retrieving near-complete genomes from RNA viruses. These findings offer promising prospects for advancing our understanding and control of infectious diseases, by facilitating detailed genomic analysis which is critical for developing targeted interventions and therapeutic strategies. | en_US |
| Affilliation | Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Eduardo de Menezes Hospital. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Central Public Health Laboratory of the State of Minas Gerais. Ezequiel Dias Foundation. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Exact and Earth Sciences. University of the State of Bahia. Salvador, BA, Brazil. | en_US |
| Affilliation | Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil/René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil/Morphogenesis and Antigenicity of HIV and Hepatitis Viruses. University of Tours. Tours, France. | en_US |
| Affilliation | Central Public Health Laboratory of the State of Minas Gerais. Ezequiel Dias Foundation. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Eduardo de Menezes Hospital. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Eduardo de Menezes Hospital. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Eduardo de Menezes Hospital. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Department of Sciences and Technologies for Sustainable Development and One Health. Università Campus Bio-Medico di Roma. Rome, Italy/Oswaldo Cruz Foundation. Rio de Janeiro, RJ, Brazil. | en_US |
| Subject | acute febrile illness | en_US |
| Subject | metagenomics | en_US |
| Subject | nanopore sequencing | en_US |
| Subject | severe neurological disorders | en_US |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
