| Author | Sousa, Thiago Alonso Stephan Lacerda | |
| Author | Almeida, Nathalie Bonatti Franco | |
| Author | Santos, Fabrício A. | |
| Author | Filgueiras, Priscilla Soares | |
| Author | Corsini, Camila Amormino | |
| Author | Lacerda, Camila Maria de Souza | |
| Author | Silva, Thais G. | |
| Author | Queiroz, Rafaella Fortini Grenfell e | |
| Author | Plentz, Flavio | |
| Access date | 2024-09-20T18:38:04Z | |
| Available date | 2024-09-20T18:38:04Z | |
| Document date | 2024 | |
| Citation | SOUSA, Thiago Alonso Stephan Lacerda et al. Ultrafast and highly sensitive detection of SARS-CoV-2 spike protein by field-effect transistor graphene-based biosensors. Nanotechnology, v. 35, n. 42, p. 1-8, 2 Aug. 2024. | |
| ISSN | 0957-4484 | |
| URI | https://www.arca.fiocruz.br/handle/icict/65989 | |
| Language | eng | en_US |
| Publisher | IOP Publishing | |
| Rights | open access | |
| Title | Ultrafast and highly sensitive detection of SARS-CoV-2 spike protein by field-effect transistor graphene-based biosensors | en_US |
| Type | Article | |
| DOI | 10.1088/1361-6528/ad67e8 | |
| Abstract | The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), etiological agent for the coronavirus disease 2019 (COVID-19), has resulted in over 775 million global infections. Early diagnosis remains pivotal for effective epidemiological surveillance despite the availability of vaccines. Antigen-based assays are advantageous for early COVID-19 detection due to their simplicity, cost-effectiveness, and suitability for point-of-care testing (PoCT). This study introduces a graphene field-effect transistor-based biosensor designed for high sensitivity and rapid response to the SARS-CoV-2 spike protein. By functionalizing graphene with monoclonal antibodies and applying short-duration gate voltage pulses, we achieve selective detection of the viral spike protein in human serum within 100 µs and at concentrations as low as 1 fg ml−1, equivalent to 8 antigen molecules per µl of blood. Furthermore, the biosensor estimates spike protein concentrations in serum from COVID-19 patients. Our platform demonstrates potential for next-generation PoCT antigen assays, promising fast and sensitive diagnostics for COVID-19 and other infectious diseases. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Física. Belo Horizonte, MG, Brazil / Technical University of Denmark. DTU Physics. Kongens Lyngby, Denmark. | |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Física. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Física. Belo Horizonte, MG, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Física. Belo Horizonte, MG, Brasil. | |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Física. Belo Horizonte, MG, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Física. Belo Horizonte, MG, Brasil. | |
| Subject | Graphene | en_US |
| Subject | Biosensor | en_US |
| Subject | Monoclonal antibody | en_US |
| Subject | Field-effect transistor | en_US |
| Subject | COVID-19 | en_US |
| Subject | SARS-CoV-2 | en_US |
| e-ISSN | 1361-6528 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
| xmlui.metadata.dc.subject.ods | 09 Indústria, inovação e infraestrutura | |
| xmlui.metadata.dc.subject.ods | 17 Parcerias e meios de implementação | |
