| Author | Valeriano, Alline Teixeira | |
| Author | Camara, Lais Santos | |
| Author | Bernardes, Vanessa de Fátima | |
| Author | Pais, Fabiano Sviatopolk-Mirsky | |
| Author | Araújo, Flávio Marcos Gomes | |
| Author | Salim, Anna Christina de Matos | |
| Author | Fernandes, Gabriel da Rocha | |
| Author | Stussi, Fernanda | |
| Author | Gomes, Carolina Cavalieri | |
| Author | Santos, Pedro Paulo de Andrade | |
| Author | Souza, Lélia Batista de | |
| Author | Gomez, Ricardo Santiago | |
| Author | Diniz, Marina Gonçalves | |
| Access date | 2024-09-20T18:55:04Z | |
| Available date | 2024-09-20T18:55:04Z | |
| Document date | 2024 | |
| Citation | VALERIANO, Alline Teixeira et al. MMP13 expression and activity suggest its role in bone resorption in ameloblastomas. Journal of Oral Pathology & Medicine, v. 53, n. 9, p. 577-583, Oct. 2024. | |
| ISSN | 0904-2512 | |
| URI | https://www.arca.fiocruz.br/handle/icict/65990 | |
| Language | eng | en_US |
| Publisher | Wiley | |
| Rights | restricted access | |
| Title | MMP13 expression and activity suggest its role in bone resorption in ameloblastomas | en_US |
| Type | Article | |
| DOI | 10.1111/jop.13577 | |
| Abstract | Background: Ameloblastoma is a locally destructive benign odontogenic tumor. While the neoplastic cells of conventional ameloblastoma can infiltrate the connective tissue and bone, in unicystic ameloblastoma the epithelium is encapsulated. The mechanisms driving ameloblastoma's bone resorption remains unclear. Methods: RNA sequencing (RNA-seq) was performed in a discovery cohort of conventional ameloblastoma, and pathway enrichment analysis was carried out. mRNA levels of MMP13, a gene associated with bone resorption, were assessed using RT-qPCR in a larger cohort of conventional ameloblastoma and in unicystic ameloblastoma. Zymogram gels and the immunoexpression profile of collagenase 3 (encoded by MMP13 gene) were evaluated as well. Results: Enriched pathways related to bone mineralization and upregulation of MMP13 were observed in ameloblastomas. Collagenolytic activity of collagenase 3 was detected in the tumor lysates. Collagenase 3 immunopositivity was observed in ameloblastomatous epithelium infiltrating the fibrous capsule of unicystic ameloblastoma. At the tumor–bone interface, collagenase 3 expression was detected in stromal cells, osteoblasts, and osteocytes. Conclusion: The results indicate a potential involvement of MMP13 in ameloblastoma-related bone resorption and progression. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Biological Sciences Institute. Department of Pathology. Belo Horizonte, MG, Brasil. | |
| Affilliation | Universidade Federal de Minas Gerais. Biological Sciences Institute. Department of Pathology. Belo Horizonte, MG, Brasil. | |
| Affilliation | Universidade Federal de Minas Gerais. Biological Sciences Institute. Department of Pathology. Belo Horizonte, MG, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | |
| Affilliation | Universidade Federal de Minas Gerais. Biological Sciences Institute. Belo Horizonte, MG, Brasil. | |
| Affilliation | Universidade Federal de Minas Gerais. Biological Sciences Institute. Department of Pathology. Belo Horizonte, MG, Brasil. | |
| Affilliation | Universidade Federal do Rio Grande do Norte. Department of Morphology. Natal, RN, Brasil. | |
| Affilliation | Universidade Federal do Rio Grande do Norte. Department of Oral Pathology. Natal, RN, Brasil. | |
| Affilliation | Universidade Federal de Minas Gerais. School of Dentistry. Department of Oral Surgery and Pathology. Belo Horizonte, MG, Brasil / Faculdade Ciências Médicas de Minas Gerais. Medical School. Belo Horizonte, MG, Brasil. | |
| Affilliation | Universidade Federal de Minas Gerais. Biological Sciences Institute. Department of Pathology. Belo Horizonte, MG, Brasil. | |
| Subject | Ameloblastoma | en_US |
| Subject | Bone resorption | en_US |
| Subject | Collagenase 3 | en_US |
| Subject | Matrix metalloproteinase 13 | en_US |
| Subject | Odontogenic tumor | en_US |
| e-ISSN | 1600-0714 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
