| Author | Bokel, Joanna | |
| Author | Gonçalves, Remy Martins | |
| Author | Grinsztejn, Eduarda | |
| Author | Mendes-de-Almeida, Daniela P. | |
| Author | Hoagland, Brenda | |
| Author | Cardoso, Sandra Wagner | |
| Author | Geraldo, Kim Mattos | |
| Author | Coutinho, Sandro Nazer | |
| Author | Georg, Ingebourg | |
| Author | Oliveira, Maria Helena | |
| Author | Souza, Flávia dos Santos | |
| Author | Sacramento, Carolina Q. | |
| Author | Rozini, Stephane Vicente | |
| Author | Vizzoni, Alexandre G. | |
| Author | Veloso, Valdiléa G. | |
| Author | Bozza, Patrícia T. | |
| Author | Grinsztejn, Beatriz | |
| Access date | 2024-09-24T20:21:57Z | |
| Available date | 2024-09-24T20:21:57Z | |
| Document date | 2024 | |
| Citation | BOKEL, Joanna et al. Anti-PF4 positivity and platelet activation after Ad26.COV2⋅S vaccination in Brazil. Vaccine, v. 42, n. 25, p. 1-8, Nov. 2024. | |
| ISSN | 0264-410X | |
| URI | https://www.arca.fiocruz.br/handle/icict/66072 | |
| Description | Produção científica do Laboratório de Imunofarmacologia. | pt_BR |
| Language | eng | en_US |
| Publisher | Elsevier | |
| Rights | open access | |
| Title | Anti-PF4 positivity and platelet activation after Ad26.COV2·S vaccination in Brazil | en_US |
| Type | Article | |
| DOI | 10.1016/j.vaccine.2024.126175 | |
| Abstract | Introduction: The Ad26.COV2·S (Janssen/Johnson & Johnson) COVID-19 vaccine, has been rarely associated with vaccine-induced immune thrombocytopenia and thrombosis (VITT). We investigated the prevalence of anti-PF4 antibody positivity, thrombocytopenia, D-dimer elevation, plasmatic thromboinflammatory markers, and platelet functional assays following Ad26.COV2·S vaccination in Rio de Janeiro, Brazil. Methods: From July to September 2021, participants were assessed prior, 1, and 3 weeks post-vaccination. Platelet count and D-dimer were measured at each visit and anti-PF4 at week 3. A positive anti-PF4 prompted retrospective testing of the sample from week 0. Individuals with new thrombocytopenia or elevated D-dimer, positive anti-PF4, and 38 matched controls without laboratory abnormalities were evaluated for plasmatic p-selectin, tissue factor, and functional platelet activation assays. Results: 630 individuals were included; 306 (48.57%) females, median age 28 years. Forty-two (6.67%) presented ≥1 laboratory abnormality in week 1 or 3. Five (0.79%) had thrombocytopenia, 31 (4.91%) elevated D-dimer, and 9 (1.57%) had positive anti-PF4 at week 3. Individuals with laboratory abnormalities and controls showed a slight increase in plasmatic p-selectin and tissue factor. Ten individuals with laboratory abnormalities yielded increased surface expression of p-selectin, and their ability to activate platelets in a FcγRIIa dependent manner was further evaluated. Two were partially inhibited by high concentrations of heparin and blockage of FcγRII with IV.3 antibody. Plasma obtained before vaccination produced similar results, suggesting a lack of association with vaccination. Conclusions: Vaccination with Ad26.COV2·S vaccine led to a very low frequency of low-titer positive anti-PF4 antibodies, elevation of D-dimer, and mild thrombocytopenia, with no associated clinically relevant increase in thromboinflammatory markers and platelet activation. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | University Hospitals. Case Western University. Cleveland, OH, USA. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Agência Transfusional. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST e AIDS. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST e AIDS. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST e AIDS. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST e AIDS. Rio de Janeiro, RJ, Brasil. | |
| Subject | COVID-19 | en_US |
| Subject | Ad26.COV2⋅S vaccine | en_US |
| Subject | Anti-PF4 antibodies | en_US |
| Subject | Platelets activation | en_US |
| Subject | Vaccineinduced thrombotic thrombocytopenia | en_US |
| Subject | Thromboinflamation | en_US |
| e-ISSN | 1873-2518 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
