Author | Salvioni Recalde, Oscar Daniel | |
Author | Soledad Rolón, Miriam | |
Author | Celeste Velázquez, Myriam | |
Author | Kowalewski, Martin M. | |
Author | Ruiz Diaz, Jorge Javier Alfonso | |
Author | Rojas de Arias, Antonieta | |
Author | Moraes, Milton Ozório | |
Author | Gomes, Harrison Magdinier | |
Author | Baêta, Bruna de Azevedo | |
Author | Cordeiro, Matheus Dias | |
Author | Vega Gómez, María Celeste | |
Access date | 2024-09-30T13:32:39Z | |
Available date | 2024-09-30T13:32:39Z | |
Document date | 2024 | |
Citation | SALVIONI RECALDE, Oscar Daniel et al. Diversity of anaplasmataceae transmitted by ticks (ixodidae) and the first molecular evidence of anaplasma phagocytophilum and candidatus anaplasma boleense in Paraguay. Microorganisms, v. 12, n. 9, p. 1-18, 14 Sept. 2024. | |
ISSN | 2076-2607 | |
URI | https://www.arca.fiocruz.br/handle/icict/66169 | |
Description | Produção científica do Laboratório de Biologia Molecular Aplicada a Micobactérias. | pt_BR |
Sponsorship | This research was funded by the Organization for the Structural Convergence in the Mercosur Region FOCEM, grant number COF N°03/11, and the National Council of Science and Technology CONACYT-Paraguay, grant numbers: 14-INV-052 and PINV18-178. O.D.S.R received funding from BECAL (“Becas Don Carlos Antonio López”) and partially financed “The APC”. | |
Language | eng | en_US |
Publisher | MDPI | |
Rights | open access | |
Title | Diversity of anaplasmataceae transmitted by ticks (ixodidae) and the first molecular evidence of anaplasma phagocytophilum and candidatus anaplasma boleense in Paraguay | en_US |
Type | Article | |
DOI | 10.3390/microorganisms12091893 | |
Abstract | Anaplasmataceae bacteria are emerging infectious agents transmitted by ticks. The aim of this study was to identify the molecular diversity of this bacterial family in ticks and hosts, both domestic and wild, as well as blood meal sources of free-living ticks in northeastern Paraguay. The bacteria were identified using PCR-HRM, a method optimized for this purpose, while the identification of ticks and their blood meal was performed using conventional PCR. All amplified products were subsequently sequenced. The bacteria detected in the blood hosts included Ehrlichia canis, Anaplasma platys, and Anaplasma phagocytophilum, Candidatus Anaplasma boleense, and Wolbachia spp., which had not been previously reported in the country. Free-living and parasitic ticks on dogs (Canis lupus familiaris) and wild armadillos (Dasypus novemcinctus) were collected and identified as Rhipicephalus sanguineus and Amblyomma spp. The species E. canis, A. platys, A. phagocytophilum, and Ca. A. boleense were detected in domestic dog ticks, and E. canis and A. platys were found for the first time in armadillos and free-living ticks. Blood feeding sources detected in free-living ticks were rodents, humans, armadillos and dogs. Results show a high diversity of tick-borne pathogens circulating among domestic and wild animals in the northeastern region of Paraguay. | en_US |
Affilliation | Center for the Development of Scientific Research. Asunción, Paraguay / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-Graduação em Biologia Celular e Molecular. Rio de Janeiro, RJ, Brasil. | |
Affilliation | Center for the Development of Scientific Research. Asunción, Paraguay. | |
Affilliation | Fundación Moisés Bertoni. Investigación y Conservación. Asunción, Paraguay. | |
Affilliation | Consejo Nacional de Investigaciones Científicas y Técnicas. Universidad Nacional del Nordeste. Centro de Ecología Aplicada del Litoral. Estación Biológica Corrientes. Corrientes, Argentina. | |
Affilliation | Center for the Development of Scientific Research. Asunción, Paraguay. | |
Affilliation | Center for the Development of Scientific Research. Asunción, Paraguay. | |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil. | |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil. | |
Affilliation | Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Departamento de Epidemiologia e Saúde Pública. Laboratório de Doenças Parasitárias. Seropédica, RJ, Brasil. | |
Affilliation | Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Departamento de Epidemiologia e Saúde Pública. Laboratório de Doenças Parasitárias. Seropédica, RJ, Brasil. | |
Affilliation | Center for the Development of Scientific Research. Asunción, Paraguay / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-Graduação em Biologia Celular e Molecular. Rio de Janeiro, RJ, Brasil. | |
Subject | Anaplasma spp. | en_US |
Subject | Ehrlichia spp. | en_US |
Subject | Wolbachia spp. | en_US |
Subject | Tick-borne diseases | en_US |
Subject | Free-living ticks | en_US |
Subject | High-resolution melting | en_US |
e-ISSN | 2076-2607 | |