| Autor | Waisberg, Michael | |
| Autor | Lobo, Francisco Pereira | |
| Autor | Cerqueira, Gustavo M. | |
| Autor | Passos, Liana Konovaloff Jannotti | |
| Autor | Carvalho, Omar dos Santos | |
| Autor | El-Sayed, Najib M. | |
| Autor | Franco, Glória Regina | |
| Data de acesso | 2013-06-25T18:11:18Z | |
| Data de disponibilização | 2013-06-25T18:11:18Z | |
| Data do publicação | 2007 | |
| Citação | WAISBERG , Michael et al. Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni. BMC Genomics. 2007 Jun 20;8:181 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/6625 | |
| Fomento | CAPES, FAPEMIG, NIH, | pt_BR |
| Idioma | eng | pt_BR |
| Direito Autoral | open access | pt_BR |
| Título | Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni | pt_BR |
| Tipo do documento | Article | pt_BR |
| Resumo em Inglês | Background: The parasitic trematode Schistosoma mansoni is one of the major causative agents of Schistosomiasis, a disease that affects approximately 200 million people, mostly in developing countries. Since much of the pathology is associated with eggs laid by the female worm, understanding the mechanisms involved in oogenesis and sexual maturation is an important step towards the discovery of new targets for effective drug therapy. It is known that the adult female worm only develops fully in the presenceof a male worm and that the rates of oviposition and maturation of eggs are significantly increased by mating. In order to study gene transcripts associated with sexual maturation and oviposition, we compared the gene expression profiles of sexually mature and immature parasites using DNA microarrays.
Results: For each experiment, three amplified RNA microarray hybridizations and their dye swaps were analyzed. Our results show that 265 transcripts are differentially expressed in adult females and 53 in adult males when mature and immature worms are compared. Of the genes differentially expressed, 55% are expressed at higher levels in paired females while the remaining 45% are more expressed in unpaired ones and 56.6% are expressed at higher levels in paired male worms while the remaining 43.4% are more expressed in immature parasites. Real-time RT-PCR analysis validated the microarray results. Several new maturation associated transcripts were identified. Genes that were up-regulated in single-sex females were mostly related to energy generation (i.e. carbohydrateand protein metabolism, generation of precursor metabolites and energy, cellularcatabolism, and organelle organizationand biogenesis) while genes that were down-regulated related to RNA metabolism, reactive oxygen species metabolism, electron transport, organelle organization and biogenesisand protein biosynthesis.
Conclusion: Our results confirm previous observations related togene expression induced by sexual maturation in female schistosome worms. They also increase the list of S. mansoni maturation associated transcripts considerably, therefore opening new and exciting avenues for the study of the conjugal biology and development of new drugs against schistosomes. | pt_BR |
| Afiliação | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Imunologia e Bioquímica. Laboratório de Genética Bioquímica. Belo Horizonte, MG, Brazil/ The Institute for Genomic Research. Department of Parasite Genomics. Rockville, MD, USA | pt_BR |
| Afiliação | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Imunologia e Bioquímica. Laboratório de Genética Bioquímica. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliação | The Institute for Genomic Research. Department of Parasite Genomics, , Rockville, MD, USA/ Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Imunologia e Bioquímica. Laboratório de Genética Molecular de Tripanosomatídeos. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliação | Fundação Osvaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliação | Fundação Osvaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil, | pt_BR |
| Afiliação | University of Maryland. Department of Cell Biology and Molecular Genetics and Center for Bioinformatics and Computational Biology. College Park, MD, USA / The Institute for Genomic Research. Department of Parasite Genomics, Rockville, MD, USA | pt_BR |
| Afiliação | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Imunologia e Bioquímica. Laboratório de Genética Bioquímica. Belo Horizonte, MG, Brazil | pt_BR |
| Palavras-chave em inglês | Schistosoma mansoni | pt_BR |
| Palavras-chave em inglês | PCR | pt_BR |
