| Author | Piffer, Alicia C. | |
| Author | Reis, Flavia C. G. | |
| Author | Moyrand, Frédérique | |
| Author | Montalvão, Bruna | |
| Author | Rodrigues, Marcio L. | |
| Author | Janbon, Guilhem | |
| Author | Rizzo, Juliana | |
| Access date | 2024-10-04T20:34:08Z | |
| Available date | 2024-10-04T20:34:08Z | |
| Document date | 2024 | |
| Citation | PIFFER, Alicia C. et al. Protocol for separation of fungal extracellular vesicles using ultracentrifugation from solid medium cultures. STAR Protocols, v. 5, n. 2, p. 1-12, Jun. 2024. | en_US |
| ISSN | 2666-1667 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/66325 | |
| Sponsorship | FAPERJ; ANR grant; Institut Pasteur. | en_US |
| Language | eng | en_US |
| Publisher | Cell Press | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Vesículas extracelulares | en_US |
| Subject in Portuguese | Fungo | en_US |
| Subject in Portuguese | Protocolo | en_US |
| Title | Protocol for separation of fungal extracellular vesicles using ultracentrifugation from solid medium cultures | en_US |
| Type | Article | en_US |
| DOI | 10.1016/j.xpro.2024.103069 | |
| Abstract | Extracellular vesicles (EVs) have been identified in diverse fungi, including human pathogens. In this protocol, we present two techniques for isolating and analyzing fungal EVs. The first is for high-throughput screening, and the second is for yielding concentrated samples suitable for centrifugation-based density gradients. We describe steps for analytical assays such as nano-flow cytometry and nanoparticle tracking analysis to measure EV dimensions and concentration. EV suspensions can serve diverse assays, including electron microscopy, compo sitional determination, and cell-to-cell communication assays. For complete details on the use and execution of this protocol, please refer to Rizzo et al.,1 Rizzo et al.,2 Reis et al.,3 and Reis et al.4. | en_US |
| Affilliation | Institut Pasteur. Université Paris Cité. Unité Biologie des ARN des Pathogénes Fongiques. Département de Mycologie. Paris, France. | en_US |
| Affilliation | Institut Pasteur. Université Paris Cité. Unité Biologie des ARN des Pathogénes Fongiques. Département de Mycologie. Paris, France. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Institut Pasteur. Université Paris Cité. Unité Biologie des ARN des Pathogénes Fongiques. Département de Mycologie. Paris, France. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Extracellular vesicles | en_US |
| Subject | Protocol | en_US |
| Subject | Fungi | en_US |
| DeCS | Vesículas Extracelulares | en_US |
| DeCS | Fungos | en_US |
