| Author | Sant'Anna, Lincoln de Oliveira | |
| Author | Sant'Anna, Louisy Sanches dos Santos | |
| Author | Ramos, Juliana Nunes | |
| Author | Bokermann, Sérgio | |
| Author | Sousa, Mireille Ângela Bernardes | |
| Author | Prates, Fernanda Diniz | |
| Author | Guaraldi, Ana Luíza Mattos | |
| Author | Vieira, Verônica Viana | |
| Author | Araújo, Max Roberto Batista de | |
| Access date | 2024-10-18T17:59:56Z | |
| Available date | 2024-10-18T17:59:56Z | |
| Document date | 2024 | |
| Citation | SANT'ANNA, Lincoln de Oliveira et al. Genotypic and phenotypic characterization of the first Latin America isolates of Corynebacterium rouxii, a recently described member of the Corynebacterium diphtheriae complex reported in Europe. Brazilian Journal of Microbiology, p. 1-9, 7 Oct. 2024. | |
| ISSN | 1517-8382 | |
| URI | https://www.arca.fiocruz.br/handle/icict/66595 | |
| Description | Produção científica do Laboratório Interdisciplinar de Pesquisas Médicas. | pt_BR |
| Sponsorship | This work was supported by Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) # E-17/205.900/2022 and # E-26/202.088/2020, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) # 309948/2018-5. | |
| Language | eng | en_US |
| Publisher | Sociedade Brasileira de Microbiologia | |
| Rights | restricted access | |
| Title | Genotypic and phenotypic characterization of the first Latin America isolates of Corynebacterium rouxii, a recently described member of the Corynebacterium diphtheriae complex reported in Europe | en_US |
| Type | Article | |
| DOI | 10.1007/s42770-024-01526-4 | |
| Abstract | The genus Corynebacterium is the largest genera among corynebacteria and has a range of species widely spread in ecological niches, some with epidemic potential and capable of causing fatal diseases. In recent years, due to the reclassifications and discoveries of new potentially toxin-producing species, microbiological identification and epidemiological control have been compromised, becoming possible only with sequencing techniques. Two bacterial strains isolated from a cat were identified by MALDI-TOF mass spectrometry as Corynebacterium diphtheriae and sent to the collaborating center of the Brazilian Ministry of Health for molecular identification and determination of toxigenicity potential, which were initially performed by multiplex PCR method. In addition, the antimicrobial susceptibility profile was determined according to BrCAST. Finally, for the final identification at the species level and effective epidemiological monitoring, the sequencing of the 16S rRNA and rpoB housekeeping genes was carried out. The isolates were identified as nontoxigenic C. diphtheriae strains by mPCR. Both strains were found susceptible to all antimicrobial agents. Although the identification at the species level was not possible through similarity analysis of S rRNA and rpoB housekeeping genes, the phylogenetic analysis showed that the isolates belonged to the species Corynebacterium rouxii with a high value of reliability. This is the first report of the isolation of C. rouxii in Latin America. Molecular identification, whether by the MALDI-TOF mass spectrometry or PCR techniques, does not discriminate C. rouxii from C. diphtheriae, requiring gene sequencing and phylogenetic analysis for correct identification at the species level. | en_US |
| Affilliation | Universidade do Estado do Rio de Janeiro. Centro Biomédico. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Difteria e Corinebactérias de Importância Clínica. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade do Estado do Rio de Janeiro. Centro Biomédico. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Difteria e Corinebactérias de Importância Clínica. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade do Estado do Rio de Janeiro. Centro Biomédico. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Difteria e Corinebactérias de Importância Clínica. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Governo do Estado de São Paulo. Secretaria de Estado da Saúde. Instituto Adolfo Lutz. Centro de Bacteriologia. São Paulo, SP, Brasil. | |
| Affilliation | Universidade do Estado do Rio de Janeiro. Centro Biomédico. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Difteria e Corinebactérias de Importância Clínica. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Hermes Pardini Institute. Microbiology. Operational Technical Nucleus. Belo Horizonte, MG, Brasil. | |
| Affilliation | Universidade do Estado do Rio de Janeiro. Centro Biomédico. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Difteria e Corinebactérias de Importância Clínica. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Hermes Pardini Institute. Microbiology. Operational Technical Nucleus. Belo Horizonte, MG, Brasil. | |
| Subject | Corynebacterium rouxii | en_US |
| Subject | C. diphtheriae complex | en_US |
| Subject | Mass spectrometry | en_US |
| Subject | Molecular identification | en_US |
| Subject | Gene sequencing | en_US |
| e-ISSN | 1678-4405 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
| xmlui.metadata.dc.subject.ods | 09 Indústria, inovação e infraestrutura | |
