| Author | Machado, Eduarda de Oliveira Silva Lima | |
| Author | Freitas, Tatiana Pádua Tavares de | |
| Author | Pinto, Isaac Leandro Lira | |
| Author | Dias, Thiago Dutra | |
| Author | Oliveira, Lais da Silva de | |
| Author | Tiepolo, Liliani Marilia | |
| Author | Santos, Huarrisson Azevedo | |
| Author | Massard, Carlos Luiz | |
| Author | Santos, Fernando de Oliveira | |
| Author | Teixeira, Bernardo Rodrigues | |
| Author | Peixoto, Maristela Peckle | |
| Access date | 2024-10-21T13:10:41Z | |
| Available date | 2024-10-21T13:10:41Z | |
| Document date | 2024 | |
| Citation | MACHADO, Eduarda de Oliveira Silva Lima et al. Detection, genetic diversity, and factors associated with infection by hemotropic mycoplasmas in rodent and marsupial populations in regions of the states of Rio de Janeiro and Parana, Brazil. Microbial Pathogenesis, p. 1-29, 17 Oct. 2024. | |
| ISSN | 0882-4010 | |
| URI | https://www.arca.fiocruz.br/handle/icict/66617 | |
| Description | Produção científica do Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. | pt_BR |
| Sponsorship | We are grateful to Universidade Federal Rural do Rio de Janeiro (UFRRJ), Instituto Oswaldo Cruz/Fundação Oswaldo Cruz (IOC/FIOCRUZ) and Universidade Federal do Paraná (UFPR) for support and financing this research and ICMBio for authorizing fieldwork. We thank Jorge Pinto da Silva, Adilson Benedito de Almeida and LABPMR/IOC/FIOCRUZ, LHV/UFRRJ and UFPR team for their support in scientific expeditions. We also thank, the state health secretaries of Rio de Janeiro and Paraná, as well as municipal health secretaries of Nova Friburgo, Cruz Machado, Lidianópolis and Ponta Grossa, and the 3rd, 6th and 22nd regional health secretaries of state of Paraná. The present study was also financed by the Carlos Chagas Filho Foundation for Research Support of the State of Rio de Janeiro (FAPERJ, Process E-26/010.0002715/2019 and FAPERJ, Process JCNE E-26/200.234/2023), by the Brazilian National Council for Scientific and Technological Development (CNPq, Process 421292/2017-2) and by the Brazilian Coordination of Higher Education Personnel Training (CAPES), through a Masters scholarship to Eduarda de Oliveira Silva Lima Machado. | |
| Language | eng | en_US |
| Publisher | Elsevier | |
| Rights | restricted access | |
| Subject in Portuguese | Hemoplasmas | pt_BR |
| Subject in Portuguese | Bactérias epicelulares | pt_BR |
| Subject in Portuguese | Pequenos mamíferos | pt_BR |
| Subject in Portuguese | Filogenia | pt_BR |
| Subject in Portuguese | Mata Atlântica | pt_BR |
| Title | Detection, genetic diversity, and factors associated with infection by hemotropic mycoplasmas in rodent and marsupial populations in regions of the states of Rio de Janeiro and Parana, Brazil | en_US |
| Type | Preprint | |
| DOI | 10.1016/j.micpath.2024.107017 | |
| Abstract | Hemotropic mycoplasmas, also known as hemoplasmas, are found in several animal species around the world. New species and genotypes of mycoplasmas have been described recently but much more remains to be discovered, especially in wildlife. The objective of this study was to investigate the presence of hemotropic mycoplasmas and diversity of the 16S rDNA sequence fragment in populations of small nonflying mammals in regions with different phytophysiognomies in the states of Rio de Janeiro and Parana, Brazil, as well as to evaluate environmental and animal factors influencing infection rates. Of the 257 animals sampled, 33.8% (n=87) were considered positive by conventional PCR. Of the 230 samples from wild rodents (Cricetidae), 36.5% (n=84) amplified hemotropic mycoplasma DNA, followed by synanthropic rodents (Muridae) (12.5%, n= 2/16), and marsupials (Didelphidae) (9.0%, n= 1/11). The regions with the highest frequency of positivity were Ponta Grossa (46.5%, n=27/58) and Cruz Machado (46.1%, n= 24/52), followed by Nova Friburgo (30.8%, n= 33/107), and Lidianopolis (12.5%, n=5/40). Oligoryzomys had the highest rate of infection (78.5%), statistically different from Oxymycterus spp. (40.0%), Akodon spp. (27.0%) and Sooretamys (8.3%). Males had a higher prevalence of Mycoplasma spp., being 3.97 times more likely to be positive for hemoplasma than females. Phylogenetic analysis grouped the sequences from this study into two clades, A and E, together with other hemoplasma sequences previously detected in small wild and synanthropic rodents from Brazil, Hungary, and Japan. Haplotype network reveals exclusive haplotypes for wild and synanthropic rodents. Our study reveals considerable genetic diversity suggesting the circulation of at least two species among sequences from this study and highlights the importance of considering environmental and biological factors in the epidemiology of these infections in wild mammals. | en_US |
| Affilliation | Federal Rural University of Rio de Janeiro. Veterinary Institute. Department of Animal Parasitology. Seropédica, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Federal Rural University of Rio de Janeiro. Veterinary Institute. Department of Animal Parasitology. Seropédica, RJ, Brasil. | |
| Affilliation | Federal Rural University of Rio de Janeiro. Veterinary Institute. Department of Animal Parasitology. Seropédica, RJ, Brasil. | |
| Affilliation | Federal Rural University of Rio de Janeiro. Veterinary Institute. Department of Animal Parasitology. Seropédica, RJ, Brasil. | |
| Affilliation | Universidade Federal do Paraná. Laboratory of Analysis and Monitoring of Atlantic Forest. Matinhos, PR, Brasil. | |
| Affilliation | Federal Rural University of Rio de Janeiro. Veterinary Institute. Department of Epidemiology and Public Health. Seropédica, RJ, Brasil. | |
| Affilliation | Federal Rural University of Rio de Janeiro. Veterinary Institute. Department of Animal Parasitology. Seropédica, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Federal Rural University of Rio de Janeiro. Veterinary Institute. Department of Animal Parasitology. Seropédica, RJ, Brasil. | |
| Subject | Hemoplasmas | en_US |
| Subject | Epicellular bacteria | en_US |
| Subject | Small mammals | en_US |
| Subject | Phylogeny | en_US |
| Subject | Atlantic Forest | en_US |
| e-ISSN | 1096-1208 | |
| Peer reviewed | Não | |
| Publication status | Não Publicado | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
| xmlui.metadata.dc.subject.ods | 15 Vida terrestre | |
