| Author | Loreiro, Júlia Maria Peixoto | |
| Author | Guimarães, Rhuan Carlos Coelho | |
| Author | Valadão, Talita Bernardo | |
| Author | Miranda, Rebeca Vitória da Silva Lage de | |
| Author | Andrade, Joyce Modesto de | |
| Author | Costa, Luciana Veloso da | |
| Author | Brandão, Marcelo Luiz Lima | |
| Access date | 2024-10-24T11:33:41Z | |
| Available date | 2024-10-24T11:33:41Z | |
| Document date | 2024 | |
| Citation | LOUREIRO, Júlia Maria Peixoto et al. Application of Fourier-transform infrared spectroscopy (FT-IR) for Staphylococcus epidermidis typing as a tool for contamination control strategy in a pharmaceutical industry facility. In: PARENTERAL DRUG ASSOCIATION (PDA) MICROBIOLOGICAL CONFERENCE, 2024, Washington D. C., 2024. 1 p. | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/66706 | |
| Language | eng | en_US |
| Publisher | PDA | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Staphylococcus epidermidis | en_US |
| Subject in Portuguese | FT-IR | en_US |
| Subject in Portuguese | Espectroscopia de Infravermelho com Transformada de Fourier | en_US |
| Subject in Portuguese | Técnicas de Tipagem Bacteriana | en_US |
| Subject in Portuguese | Controle de contaminação | en_US |
| Subject in Portuguese | Indústria Farmacêutica | en_US |
| Title | Application of Fourier-transform infrared spectroscopy (FT-IR) for Staphylococcus epidermidis typing as a tool for contamination control strategy in a pharmaceutical industry facility | en_US |
| Type | Papers presented at events | en_US |
| Abstract | Abstract Overview: The typing of micro-organisms in pharmaceutical factories often relies on expensive and time-consuming molecular techniques. So, the implementation of cheap, fast and reliable typing methods in the routine would speed up the investigation procedures improving the contamination control strategy. The Fourier-transform infrared (FT-IR) spectroscopy is a method that generates spectra, that enables to micro-organisms typing within 3 h. This study aimed to evaluate the FT-IR for typing S. epidermidis strains isolated from an immunobiological pharmaceutical industry in Brazil. Fifty strains were evaluated by FT-IR using IR Biotyper®. A dendrogram was created with the raw data to cluster the separation spectrum and the cut-off value was automatically calculated. Forty-four FT-IR profiles were obtained, a ratio of 1.14 strain/profile. From the five clusters formed, Cluster 1, 2 and 3 (6 strains) were isolated from environmental monitoring of air and operators (EMO). Cluster 4 (3 strains) were isolated from EMO and bioburden assays, suggesting that the environment could be the main source of bacterial contamination in the product analyzed in bioburden assay. Cluster 5 (2 strains) were isolated from EMO and a cell culture lineage used in quality control assays, suggesting that the environment could also be the main source of cell contamination. Learning Objectives: FT-IR using IR Biotyper® seems to be a promising technique for S. epidermidis strains typing and tracking possible sources of contamination in the immunobiological facility evaluated in this study. This equipment allows the creation of a specific database for each bacterial species, which can be updated continuously and used for analysis by time space of specific product groups and/or areas in the facility, among others. The methodology applied in this study can be used as part of the contamination control strategy to develop preventive and corrective measures to eliminate microbial contamination. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos). Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | Staphylococcus epidermidis | en_US |
| Subject | FT-IR | en_US |
| Subject | Spectroscopy, Fourier Transform Infrared | en_US |
| Subject | Bacterial Typing Techniques | en_US |
| DeCS | Técnicas de Tipagem Bacteriana | en_US |
| DeCS | Espectroscopia de Infravermelho com Transformada de Fourier | en_US |
| DeCS | Staphylococcus epidermidis | en_US |
| DeCS | Indústria Farmacêutica | en_US |
