| Author | Drescher, Guilherme | |
| Author | Santos, Hellen Geremias | |
| Author | Pinto, Mariane Marques da Guarda | |
| Author | Morello, Luis Gustavo | |
| Author | Figueiredo, Fabiano Borges | |
| Access date | 2024-10-26T21:25:53Z | |
| Available date | 2024-10-26T21:25:53Z | |
| Document date | 2024 | |
| Citation | DRESCHER, Guilherme et al. Diagnosis of fasciolosis antibodies in Brazilian cattle through ELISA employing both native and recombinant antigens. Microbiology Spectrum, v. 12, n. 5, p. 1-14, May 2024. | en_US |
| ISSN | 2165-0497 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/66751 | |
| Description | Supplemental Material
Figure S1 (Spectrum00095-24-S0001. Distribution of absorbance values.
Figure S2 (Spectrum00095-24-S0002. ROC curves.
Tables S1 and S2 (Spectrum00095-24-S0003.docx). Diagnostic performance measures for the three tests on farms cattle (n = 500, 38 positive cases). | en_US |
| Sponsorship | CNPq, CAPES | en_US |
| Language | eng | en_US |
| Publisher | American Society for Microbiology | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | ELISA | en_US |
| Subject in Portuguese | Fasciolose | en_US |
| Subject in Portuguese | Gado | en_US |
| Subject in Portuguese | Antígenos nativos (FhES and FhSA) | en_US |
| Subject in Portuguese | Antígenos recombinantes (FhrCL-1) | en_US |
| Title | Diagnosis of fasciolosis antibodies in Brazilian cattle through ELISA employing both native and recombinant antigens | en_US |
| Type | Article | en_US |
| DOI | 10.1128/spectrum.00095-24 | |
| Abstract | Bovine fasciolosis is a parasitic disease with a global reach. Coprological based on egg detection in fecal samples and liver inspection to evaluate the presence of the parasite is currently the gold standard for diagnosing chronic fasciolosis in cattle. However, these techniques are labor-intensive and ineffective during the acute phase of the disease. Serodiagnosis using native and recombinant antigens has become an interesting alternative in efforts to identify cattle fasciolosis. We evaluated cattle from abattoir (n = 139) and farms (n = 500) through liver inspection and coprological examination, respectively. Our laboratory team optimized and validated enzyme-linked immunosorbent assay tests based on somatic antigen, excretory/secretory proteins, and the recombinant antigen cathepsin L-1 to detect serum antibodies against fasciolosis in cattle. For animals from abattoir, 10 were positive for fasciolosis according to liver inspection. Both FhES and FhrCL-1 presented an area under the receiver operating characteristic (AUROC) curve of 0.80, with a sensitivity of 0.80 (95% CI: 0.46–0.95) and 0.70 (95% CI: 0.38–0.90) and specificity of 0.81 (95% CI: 0.73–0.87) and 0.87 (95% CI: 0.80–0.92), respectively. For those cattle from farms, 28 were positive only for fasciolosis according to coprological examination. In this scenario, FhES gave the best performance, with an AUROC of 0.84, sensitivity of 0.79 (95% CI: 0.60–0.90), and specificity of 0.86 (95% CI: 0.82–0.89). In conclusion, our study highlights the potential of serodiagnosis for accurately screening cattle fasciolosis. The promising sensitivity and specificity values of FhES when compared to liver inspection or coprological examination enhance its importance for cattle fasciolosis diagnosis. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil. | en_US |
| Subject | Fasciolosis | en_US |
| Subject | Cattle | en_US |
| Subject | Native antigens (FhES and FhSA) | en_US |
| Subject | Recombinant antigen (FhrCL-1) | en_US |
| Subject | ELISA | en_US |
| DeCS | Ensaio de Imunoadsorção Enzimática | en_US |
| DeCS | Fasciolíase | en_US |
| DeCS | Gado | en_US |
