| Author | Barros, Janaína F. | |
| Author | Waclawiak, Ingrid | |
| Author | Pecli, Cyntia | |
| Author | Borges, Paula A. | |
| Author | Georgii, Janaína L. | |
| Author | Ramos-Junior, Erivan S. | |
| Author | Canetti, Claudio | |
| Author | Courau, Tristan | |
| Author | Klatzmann, David | |
| Author | Kunkel, Steven L. | |
| Author | Penido, Carmen | |
| Author | Canto, Fábio B. | |
| Author | Benjamim, Claudia F. | |
| Access date | 2024-11-04T09:37:40Z | |
| Available date | 2024-11-04T09:37:40Z | |
| Document date | 2019 | |
| Citation | BARROS, Janaína F.; WACLAWIAK, Ingrid; PECLI, Cyntia; BORGES, Paula A.; GEORGII, Janaína L.; RAMOS-JUNIOR, Erivan S.; CANETTI, Claudio; COURAU, Tristan; KLATZMANN, David; KLATZMANN, David; KUNKEL, Steven L.; PENIDO, Carmen; PENIDO, Carmen; CANTO, Fábio B.; CANTO, Fábio B.; BENJAMIM, Claudia F.; BENJAMIM, Claudia F. Role of Chemokine Receptor CCR4 and Regulatory T Cells in Wound Healing of Diabetic Mice. Journal of Investigative Dermatology, United States, v. 139, n. 5, p. 1161-1161, 2019. DOI: 10.1016/J.JID.2018.10.039. | en_US |
| ISSN | 0022-202X | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/66901 | |
| Abstract in Portuguese | A cicatrização de feridas é um processo bem coordenado que envolve mediadores inflamatórios e respostas celulares; no entanto, se houver alguma perturbação durante esse processo, o reparo do tecido é prejudicado. Feridas crônicas são uma das complicações graves de longo prazo associadas ao diabetes mellitus. O receptor de quimiocina CCR4 e seus respectivos ligantes, CCL17 e CCL22, estão envolvidos no recrutamento e ativação de células T reguladoras na pele inflamada; no entanto, o papel das células T reguladoras em feridas ainda não está claro. Nosso objetivo foi investigar o papel do CCR4 e das células T reguladoras na cicatrização de feridas cutâneas em camundongos diabéticos. Camundongos selvagens diabéticos induzidos por aloxana (diabéticos) desenvolveram feridas difíceis de cicatrizar, diferentemente de camundongos diabéticos CCR4-/- (CCR4-/- diabéticos) e também de camundongos diabéticos injetados com anti-CCL17/22 ou anti-CD25 que apresentaram cicatrização acelerada de feridas e menos células T reguladoras no leito da ferida. Consequentemente, camundongos diabéticos CCR4-/- também apresentaram alteração na população de células T na ferida e nos linfonodos de drenagem; no dia 14, esses camundongos também apresentaram aumento na deposição de fibras de colágeno. Ainda assim, os níveis de citocina foram diminuídos nas feridas de camundongos diabéticos CCR4-/- no dia 2. Nossos dados sugerem que o receptor CCR4 e as células T reguladoras afetam negativamente a cicatrização de feridas em camundongos diabéticos. | en_US |
| Language | eng | en_US |
| Publisher | Elsevier B.V. | en_US |
| Rights | open access | en_US |
| MeSH | Alloxan | en_US |
| MeSH | Analysis of Variance | en_US |
| MeSH | Animals | en_US |
| MeSH | Biopsy, Needle | en_US |
| MeSH | Chemokine CCL17 | en_US |
| MeSH | Chemokine CCL22 | en_US |
| MeSH | Chemokines | en_US |
| MeSH | Diabetes Mellitus, Experimental | en_US |
| MeSH | Diabetes Mellitus, Type 1 | en_US |
| MeSH | Humans | en_US |
| MeSH | Immunohistochemistry | en_US |
| MeSH | Mice | en_US |
| MeSH | Mice, Inbred C57BL | en_US |
| MeSH | Mice, Knockout | en_US |
| MeSH | Real-Time Polymerase Chain Reaction | en_US |
| MeSH | Receptors, CCR4 | en_US |
| MeSH | Wound Healing | en_US |
| Subject in Portuguese | macrófago | en_US |
| Subject in Portuguese | alvos | en_US |
| Subject in Portuguese | memória | en_US |
| Subject in Portuguese | reparo | en_US |
| Title | Role of Chemokine Receptor CCR4 and Regulatory T Cells in Wound Healing of Diabetic Mice | en_US |
| Type | Article | en_US |
| DOI | 10.1016/J.JID.2018.10.039 | |
| Abstract | Wound healing is a well-coordinated process that involves inflammatory mediators and cellular responses; however, if any disturbances are present during this process, tissue repair is impaired. Chronic wounds are one of the serious long-term complications associated with diabetes mellitus. The chemokine receptor CCR4 and its respective ligands, CCL17 and CCL22, are involved in regulatory T cell recruitment and activation in inflamed skin; however, the role of regulatory T cells in wounds is still not clear. Our aim was to investigate the role of CCR4 and regulatory T cells in cutaneous wound healing in diabetic mice. Alloxan-induced diabetic wild- type mice (diabetic) developed wounds that were difficult to heal, differently from CCR4-/- diabetic mice (CCR4-/- diabetic), and also from anti-CCL17/22 or anti-CD25-injected diabetic mice that presented with accelerated wound healing and fewer regulatory T cells in the wound bed. Consequently, CCR4-/- diabetic mice also presented with alteration on T cells population in the wound and draining lymph nodes; on day 14, these mice also displayed an increase of collagen fiber deposition. Still, cytokine levels were decreased in the wounds of CCR4-/- diabetic mice on day 2. Our data suggest that the receptor CCR4 and regulatory T cells negatively affect wound healing in diabetic mice. | en_US |
| Affilliation | Institute of Biomedical Sciences, Pharmacology and Inflammation Program, Federal University of Rio de Janeiro, Center for Health Sciences, Rio de Janeiro, Brazil | en_US |
| Affilliation | Institute of Biophysics Carlos Chagas Filho, Immunobiology Program, Federal University of Rio de Janeiro, Center for Health Sciences, Rio de Janeiro, Brazil | en_US |
| Affilliation | Institute of Biomedical Sciences, Pharmacology and Inflammation Program, Federal University of Rio de Janeiro, Center for Health Sciences, Rio de Janeiro, Brazil | en_US |
| Affilliation | Institute of Biomedical Sciences, Pharmacology and Inflammation Program, Federal University of Rio de Janeiro, Center for Health Sciences, Rio de Janeiro, Brazil | en_US |
| Affilliation | Institute of Biomedical Sciences, Pharmacology and Inflammation Program, Federal University of Rio de Janeiro, Center for Health Sciences, Rio de Janeiro, Brazil | en_US |
| Affilliation | Institute of Biophysics Carlos Chagas Filho, Immunobiology Program, Federal University of Rio de Janeiro, Center for Health Sciences, Rio de Janeiro, Brazil | en_US |
| Affilliation | Institute of Biophysics Carlos Chagas Filho, Immunobiology Program, Federal University of Rio de Janeiro, Center for Health Sciences, Rio de Janeiro, Brazil | en_US |
| Affilliation | Sorbonne Universités, University of Pierre and Madam Curie, University of Paris, Paris, France | en_US |
| Affilliation | Sorbonne Universités, University of Pierre and Madam Curie, University of Paris, Paris, France / Institut National de la Santé et de la Recherche Médicale les Unités Mixtes de Recherche S959, Paris, France | en_US |
| Affilliation | Department of Pathology, University of Michigan, Ann Arbor, United States | en_US |
| Affilliation | Center for Technological Development in Health, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil / Laboratory of Applied Pharmacology, Institute of Drug Technology, Farmanguinhos, Rio de Janeiro, Brazil | en_US |
| Affilliation | Institute of Microbiology Paulo de Góes, Immunology Department, Federal University of Rio de Janeiro, Center for Health Sciences, Rio de Janeiro, Brazil / Department of Immunobiology, Institute of Biology, Fluminense Federal University, Niterói, Brazil | en_US |
| Affilliation | Institute of Biomedical Sciences, Pharmacology and Inflammation Program, Federal University of Rio de Janeiro, Center for Health Sciences, Rio de Janeiro, Brazil / Institute of Biophysics Carlos Chagas Filho, Immunobiology Program, Federal University of Rio de Janeiro, Center for Health Sciences, Rio de Janeiro, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças de Populações Negligenciadas. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | macrophage | en_US |
| Subject | targets | en_US |
| Subject | repair | en_US |
| Subject | memory | en_US |
| e-ISSN | 1523-1747 | |
