| Author | Zomer, Helena Debiazi | |
| Author | Lima, Victor Juan de Souza | |
| Author | Bion, Monique Coelho | |
| Author | Brito, Karynne Nazare Lins | |
| Author | Rode, Michele | |
| Author | Stimamiglio, Marco Augusto | |
| Author | Jeremias, Talita da Silva | |
| Author | Trentin, Andrea Gonçalves | |
| Access date | 2024-11-12T13:16:47Z | |
| Available date | 2024-11-12T13:16:47Z | |
| Document date | 2024 | |
| Citation | ZOMER, H. D. et al. Evaluation of secretomes derived from human dermal and adipose tissue mesenchymal stem/stromal cells for skin wound healing: not as effective as cells. Stem Cell Research & Therapy, v. 15, n. 15, p. 1-14, 2024. | en_US |
| ISSN | 1757-6512 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/67092 | |
| Description | The online version contains supplementary material available at https://doi.org/ 10.1186/ s13287- 023- 03630-y. | en_US |
| Sponsorship | CNPq, CAPES, Wound Healing Society (HDZ) | en_US |
| Language | eng | en_US |
| Publisher | BMC | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | MSC | en_US |
| Subject in Portuguese | Derme | en_US |
| Subject in Portuguese | Exossomo | en_US |
| Subject in Portuguese | Matrix Integra | en_US |
| Subject in Portuguese | Proteômica | en_US |
| Subject in Portuguese | Meio condicionado | en_US |
| Title | Evaluation of secretomes derived from human dermal and adipose tissue mesenchymal stem/stromal cells for skin wound healing: not as efective as cells | en_US |
| Type | Article | en_US |
| DOI | 10.1186/s13287-023-03630-y | |
| Abstract | Background Although the paracrine efects of mesenchymal stem/stromal cells (MSCs) have been recognized as crucial mediators of their regenerative efects on tissue repair, the potential of MSC secretomes as efective substi tutes for cellular therapies remains underexplored.
Methods In this study, we compared MSCs from the human dermis (DSCs) and adipose tissue (ASCs) with their secretomes regarding their efcacy for skin wound healing using a translationally relevant murine model.
Results Proteomic analysis revealed that while there was a substantial overlap in protein composition between DSC and ASC secretomes, specifc proteins associated with wound healing and angiogenesis were diferentially expressed. Despite a similar angiogenic potential in vivo, DSC and ASC secretomes were found to be less efective than cells in accelerating wound closure and promoting tissue remodeling.
Conclusions Overall, secretome-treated groups showed intermediary results between cells- and control-treated (empty scafold) groups. These fndings highlight that although secretomes possess therapeutic potential, their efcacy might be limited compared to cellular therapies. This study contributes to the growing understanding of MSC secretomes, emphasizes the need for further protocol optimization, and ofers insights into their potential applica tions in regenerative medicine. | en_US |
| Affilliation | University of Florida. Department of Physiological Sciences. Gainesville, United States of America / Universidade Federal de Santa Catarina. Departamento de Biologia Celular, Embriologia e Genética. Florianópolis, SC, Brasil. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Biologia Celular, Embriologia e Genética. Florianópolis, SC, Brasil. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Biologia Celular, Embriologia e Genética. Florianópolis, SC, Brasil / Universidade Federal do Rio de Janeiro. Instituto Nacional de Neurociência Translacional. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Biologia Celular, Embriologia e Genética. Florianópolis, SC, Brasil. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Biologia Celular, Embriologia e Genética. Florianópolis, SC, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células-tronco. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Biologia Celular, Embriologia e Genética. Florianópolis, SC, Brasil. | en_US |
| Affilliation | Universidade Federal de Santa Catarina. Departamento de Biologia Celular, Embriologia e Genética. Florianópolis, SC, Brasil / Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | MSC | en_US |
| Subject | Dermis | en_US |
| Subject | Conditioned medium | en_US |
| Subject | Exosomes | en_US |
| Subject | Integra matrix | en_US |
| Subject | Proteomic | en_US |
| DeCS | Proteômica | en_US |
| DeCS | Complexo Multienzimático de Ribonucleases do Exossomo | en_US |
