| Author | Souza, Thayana Cruz de | |
| Author | Schwarz, Marcos Gustavo Araujo | |
| Author | Silva, Daniela Marinho da | |
| Author | Maia, Carolina Rabelo | |
| Author | Araújo, Cláudia Patrícia Mendes de | |
| Author | Balieiro, Antônio Alcirley da Silva | |
| Author | Oliveira, Luiz Antonio de | |
| Author | Degrave, Wim Maurits Sylvain | |
| Author | Fernandes, Ormezinda Celeste Cristo | |
| Author | Lima, Leila de Mendonça | |
| Access date | 2024-11-21T16:28:32Z | |
| Available date | 2024-11-21T16:28:32Z | |
| Document date | 2024 | |
| Citation | SOUZA, Thayana Cruz de et al. Penicillium citrinum CFAM 521 isolated from the Amazon region: a novel source of a fibrinolytic enzyme. International Journal of Microbiology, v. 2024, n. 5306083, p. 1-12, 29 Oct. 2024. | |
| ISSN | 1687-918X | |
| URI | https://www.arca.fiocruz.br/handle/icict/67186 | |
| Description | Produção científica do Laboratório de Biologia Molecular Aplicada a Micobactérias. | pt_BR |
| Description | Produção científica do Laboratório de Genômica Aplicada e Bioinovações. | pt_BR |
| Sponsorship | The authors are grateful to the Amazonas State Research Foundation (FAPEAM) for their financial support (Microbial proteases: production, properties, and biotechnological application in health/PPSUS) and the Foundation to support Fiocruz (FIOTEC) for the scholarship granted. | |
| Language | eng | en_US |
| Publisher | Wiley | |
| Rights | open access | |
| Title | Penicillium citrinum CFAM 521 isolated from the Amazon region: a novel source of a fibrinolytic enzyme | en_US |
| Type | Article | |
| DOI | 10.1155/2024/5306083 | |
| Abstract | Fibrinolytic agents are essential in treating thrombosis, playing a critical role in improving survival rates in cardiovascular diseases. Microbial fibrinolytic proteases have emerged as promising alternatives due to their affordability, specificity, lower toxicity, and reduced side effects. Consequently, the search for microorganisms capable of producing these enzymes has gained significant economic importance in the pharmaceutical industry. This study reports and characterizes a novel fibrinolytic enzyme produced by Penicillium citrinum CFAM 521, a strain isolated from the Amazon region. The enzyme was purified using a polyethylene glycol (PEG)–phosphate salt aqueous two-phase system (ATPS). The effects of PEG molecular weight, PEG concentration, and phosphate concentration on the protease partition coefficient (K) were evaluated through a 2² full factorial design. The enzyme exhibited both fibrinolytic and fibrinogenolytic activities. After partitioning in a two-phase system with 10% (w/w) PEG and 15% (w/w) sodium phosphate, the fibrinolytic proteases were predominantly retained in the salt-rich bottom phase (K = 0.33). The enzyme has a molecular weight of 34 kDa, with optimal pH and temperature at 9°C and 37°C, respectively. Inhibitory analysis confirmed that it is a serine protease, and its activity was enhanced by the addition of Mn²⁺. Notably, the enzyme exhibited no hemolytic activity. Therefore, P. citrinum CFAM 521 represents a novel source of fibrinolytic enzymes, highlighting its potential as an alternative for the development of thrombolytic agents. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil. | |
| Affilliation | Ministério da Ciência, Tecnologia e inovações. Instituto Nacional de Pesquisas da Amazônia. Manaus, AM, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Aplicada e Bioinovações. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil. | |
| Subject | Fibrinolytic enzyme | en_US |
| Subject | PEG/phosphate | en_US |
| Subject | Penicillium | en_US |
| Subject | Thrombolytic activity | en_US |
| e-ISSN | 1687-9198 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
| xmlui.metadata.dc.subject.ods | 09 Indústria, inovação e infraestrutura | |
