| Author | Carneiro, Diego C. | |
| Author | Rocha, Vinícius P. C. | |
| Author | Damasceno, Patrícia K. F. | |
| Author | Barbosa, Josiane D. V. | |
| Author | Soares, Milena B. P. | |
| Access date | 2024-11-22T12:37:12Z | |
| Available date | 2024-11-22T12:37:12Z | |
| Document date | 2024 | |
| Citation | CARNEIRO, Diego C.; ROCHA, Vinícius P. C.; DAMASCENO, Patrícia K. F.; BARBOSA, Josiane D. V.; SOARES, Milena B. P. Therapeutic applications of synthetic gene/genetic circuits: a patent review. Frontiers in Bioengineering and Biotechnology, v. 12, p. 1-19, 2024. | en_US |
| ISSN | 2296-4185 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/67217 | |
| Sponsorship | Inova Fiocruz/Fundação Oswaldo Cruz.
Instituto Nacional de Terapia Gênica (INCT INTERGEN).
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). | en_US |
| Language | eng | en_US |
| Publisher | Frontiers Media | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Biologia sintética | en_US |
| Subject in Portuguese | Engenharia genética | en_US |
| Subject in Portuguese | Redes genéticas | en_US |
| Subject in Portuguese | Propriedade intelectual | en_US |
| Subject in Portuguese | Molecular | en_US |
| Subject in Portuguese | Circuitos | en_US |
| Subject in Portuguese | Biossensores | en_US |
| Subject in Portuguese | Interruptor genético | en_US |
| Title | Therapeutic applications of synthetic gene/genetic circuits: a patent review | en_US |
| Type | Article | en_US |
| DOI | 10.3389/fbioe.2024.1425529 | |
| Abstract | A significant limitation of numerous current genetic engineering therapy approaches is their limited control over the strength, timing, or cellular context of their therapeutic effect. Synthetic gene/genetic circuits are synthetic biology approaches that can control the generation, transformation, or depletion of a specific DNA, RNA, or protein and provide precise control over gene expression and cellular behavior. They can be designed to perform logical operations by carefully selecting promoters, repressors, and other genetic components. Patent search was performed in Espacenet, resulting in 38 selected patents with 15 most frequent international classifications. Patent embodiments were categorized into applications for the delivery of therapeutic molecules, treatment of infectious diseases, treatment of cancer, treatment of bleeding, and treatment of metabolic disorders. The logic gates of selected genetic circuits are described to comprehensively demonstrate their therapeutic applications. Synthetic gene circuits can be customized for precise control of therapeutic interventions, leading to personalized therapies that respond specifically to individual patient needs, enhancing treatment efficacy and minimizing side effects. They can be highly sensitive biosensors that provide real-time therapy by accurate monitoring various biomarkers or pathogens and appropriately synthesizing a therapeutic molecule. Synthetic gene circuits may also lead to the development of advanced regenerative therapies and to implantable biodevices that produce on-demand bioactive molecules. However, this technology faces challenges for commercial profitability. The genetic circuit designs need adjustments for specific applications, and may have disadvantages like toxicity from multiple regulators, homologous recombination, context dependency, resource overuse, and environmental variability. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Centro Universitário SENAI CIMATEC. Instituto SENAI de Inovação em Sistemas Avançados de Saúde. Salvador, BA, Brasil. | en_US |
| Affilliation | Centro Universitário SENAI CIMATEC. Instituto SENAI de Inovação em Sistemas Avançados de Saúde. Salvador, BA, Brasil. | en_US |
| Affilliation | Centro Universitário SENAI CIMATEC. Instituto SENAI de Inovação em Sistemas Avançados de Saúde. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Centro Universitário SENAI CIMATEC. Instituto SENAI de Inovação em Sistemas Avançados de Saúde. Salvador, BA, Brasil. | en_US |
| Subject | Synthetic biology | en_US |
| Subject | Genetic engineering | en_US |
| Subject | Gene networks | en_US |
| Subject | Intellectual property | en_US |
| Subject | Molecular | en_US |
| Subject | Circuits | en_US |
| Subject | Biosensors | en_US |
| Subject | Genetic switch | en_US |
| DeCS | Biologia sintética | en_US |
| DeCS | Engenharia genética | en_US |
| DeCS | Propriedade intelectual | en_US |
| DeCS | Técnicas biossensoriais | en_US |
