| Author | Andreolla, Ana Paula | |
| Author | Borges, Alessandra Abel | |
| Author | Nagashima, Seigo | |
| Author | Paula, Caroline Busatta Vaz | |
| Author | Noronha, Lucia | |
| Author | Zanchin, Nilson I.T | |
| Author | Bordignon, Juliano | |
| Author | Santos, Claudia Nunes Duarte | |
| Access date | 2024-12-04T12:30:24Z | |
| Available date | 2024-12-04T12:30:24Z | |
| Document date | 2024 | |
| Citation | ANDREOLLA, A. P. et al. Development of monoclonal antibodies against oropouche virus and its applicability to immunohistochemical diagnosis. Virology Journal, v. 21, n. 81, p. 1-13, 2024. | en_US |
| ISSN | 1743-422X | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/67395 | |
| Sponsorship | CNPq, CAPES | en_US |
| Language | eng | en_US |
| Publisher | BMC | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Virus oropouche | en_US |
| Subject in Portuguese | Vírus negligenciado | en_US |
| Subject in Portuguese | Anticorpo monoclonal | en_US |
| Subject in Portuguese | Ensaio de imunofluorescência indireta | en_US |
| Subject in Portuguese | Diagnóstico imuno-histoquímico | en_US |
| Title | Development of monoclonal antibodies against oropouche virus and its applicability to immunohistochemical diagnosis | en_US |
| Type | Article | en_US |
| DOI | 10.1186/s12985-024-02323-z | |
| Abstract | Orthobunyavirus oropouche ense virus (OROV), the causative agent of Oropouche fever, is widely dispersed in Brazil and South America, causing sporadic outbreaks. Due to the similarity of initial clinical symptoms caused by OROV with other arboviruses found in overlapping geographical areas, differential diagnosis is challenging. As for most neglected tropical diseases, there is a shortage of reagents for diagnosing and studying OROV pathogenesis. We therefore developed and characterized mouse monoclonal antibodies and, one of them recognizes the OROV nucleocapsid in indirect immunofluorescent (IFA) and immunohistochemistry (IHC) assays. Considering that it is the first monoclonal antibody produced for detecting OROV infections, we believe that it will be useful not only for diagnostic purposes but also for performing serological surveys and epidemiological surveillance on the dispersion and prevalence of OROV in Brazil and South America. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. | en_US |
| Affilliation | Universidade Federal de Alagoas. Instituto de Ciências Biológicas e da Saúde. Laboratório de Pesquisas em Virologia e Imunologia. Maceió, AL, Brasil. | en_US |
| Affilliation | Pontifícia Universidade Católica do Paraná. Laboratório de Patologia Experimental. Curitiba, PR, Brasil. | en_US |
| Affilliation | Pontifícia Universidade Católica do Paraná. Laboratório de Patologia Experimental. Curitiba, PR, Brasil. | en_US |
| Affilliation | Pontifícia Universidade Católica do Paraná. Laboratório de Patologia Experimental. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Estrutural e Engenharia de Proteínas. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. | en_US |
| Subject | Oropouche virus | en_US |
| Subject | Neglected virus | en_US |
| Subject | Monoclonal antibody | en_US |
| Subject | Immune-histochemistry diagnosis | en_US |
| Subject | Indirect immunofluorescent assay | en_US |
| DeCS | Infecções por Bunyaviridae | en_US |
| DeCS | Imuno-Histoquímica | en_US |
| DeCS | Anticorpos Monoclonais | en_US |
| DeCS | Técnica Indireta de Fluorescência para Anticorpo | en_US |
