| Author | Disch, Jolande | |
| Author | Pedras, Mariana Junqueira | |
| Author | Orsini, Marcela | |
| Author | Pirmez, Claude | |
| Author | Oliveira, Maria Cláudia de | |
| Author | Castro, Marcelo | |
| Author | Rabello, Ana Lúcia Teles | |
| Access date | 2013-08-05T13:59:52Z | |
| Available date | 2013-08-05T13:59:52Z | |
| Document date | 2005 | |
| Citation | DISCH, Jolande et al. Leishmania (Viannia) subgenus kDNA amplification for the diagnosis of mucosal leishmaniasis. Diagn. Microbiol. Infect. Dis., v. 51, n. 3, p. 185-190, Mar. 2005. | pt_BR |
| ISSN | 0732-8893 | |
| URI | https://www.arca.fiocruz.br/handle/icict/6743 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Title | Leishmania (Viannia) subgenus kDNA amplification for the diagnosis of mucosal leishmaniasis | pt_BR |
| Type | Article | |
| Abstract | The utility of 2 polymerase chain reaction (PCR)–based assays amplifying genus or Viannia subgenus Leishmania minicircle kDNA for the diagnostics of ML was assessed. The Viannia subgenus product was yielded after PCR from isolates of L. (Viannia) braziliensis, L. (Viannia) colombiensis, and L. (Viannia) guyanensis, whereas no product was obtained with the non-Viannia–pertaining species: L. (Leishmania) amazonensis, L. (Leishmania) donovani, and L. (Leishmania) chagasi. With both assays, 11 of 13 (86.4%) patients with confirmed ML could be identified, whereas only 2 (16.7%) of these patients were positive by microscopy. All amplified genus-specific products gave a positive signal by hybridization with a Leishmania (Viannia) subgenus–specific radioactive probe. The Vianniasubgenus–specific kDNA PCR represents a sensitive and specific tool for the diagnosis of ML, remarkably improving the sensitivity of parasitological methods and offering an alternative for the radioactive-dependent assays for subgenus characterization | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, MG, Brazil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Department of Biochemistry and Molecular Biology. Rio de Janeiro, RJ, Brazil. | pt_BR |
| Affilliation | Santa Casa de Belo Horizonte. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Internal Medicine Department. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, MG, Brazil. | pt_BR |
| Subject | Mucosal leishmaniasis | pt_BR |
| Subject | PCR | pt_BR |
| Subject | Leishmania(Viannia) subgenus | pt_BR |
