| Author | Fonseca, Cristina Toscano | |
| Author | Cunha Neto, Edécio | |
| Author | Goldberg, Anna Carla | |
| Author | Kalil, Jorge | |
| Author | Jesus, Amélia R. de | |
| Author | Carvalho, Edgard M. | |
| Author | Oliveira, Rodrigo Correa de | |
| Author | Oliveira, Sérgio Costa | |
| Access date | 2013-08-05T15:54:34Z | |
| Available date | 2013-08-05T15:54:34Z | |
| Document date | 2005 | |
| Citation | FONSECA, Cristina Toscano et al. Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis. Microbes Infect., v. 7, n. 2, p. 204-212, Feb. 2005. | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/6745 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Human T cell epitope mapping of the Schistosoma mansoni 14-kDa fatty acid-binding protein using cells from patients living in areas endemic for schistosomiasis | pt_BR |
| Type | Article | |
| Abstract | The development of a defined anti-schistosomiasis vaccine would contribute to the current control strategy mainly because immunization provides long-lasting immunity to the disease. Sm14, one of the six Schistosoma mansoni antigens selected by WHO as a candidate to compose a subunit vaccine against schistosomiasis, has been associated with resistance to S. mansoni infection in human beings and is able to induce protection in the murine model. To identify human T cell epitopes in Sm14, we used the TEPITOPE algorithm to select peptides that would most likely bind to several HLA-DR molecules. In this study, three Sm14 epitopes were selected and produced as synthetic peptides. Human T cell responses from schistosomiasis patients living in endemic areas in Brazil were determined by proliferation assay and IL-5 and IFN-γ measurements. Differential peptide recognition and cytokine production in response to Sm14 epitopes were observed in individuals resistant to S. mansoni infection versus susceptible individuals. Sm14(32-48) and Sm14(53-69) peptides were preferentially recognized by peripheral blood mononuclear cells (PBMCs) of S. mansoni-resistant individuals, and Sm14(53-69) induced significant production of IFN-γ. Additionally, Sm14(32-48) and Sm14(53-69) were “promiscuous” peptides, since they were able to induce cellular immune responses in individuals carrying 10 and 8, respectively, of the 11 HLA-DR molecules expressed in the studied population. Among Sm14 synthetic peptides tested in this study, we identified Sm14(32-48) and Sm14(53-69) as promising candidates to compose an anti-schistosomiasis vaccine, since they seem to be related to resistance to human schistosomiasis. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Investigação em Imunologia-Instituto do Milênio. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Universidade de São Paulo. Laboratório de Imunologia, Instituto do Coração. Sao Paulo, SP, Brazil. | pt_BR |
| Affilliation | Universidade de São Paulo. Laboratório de Imunologia, Instituto do Coração. Sao Paulo, SP, Brazil. | pt_BR |
| Affilliation | Universidade de São Paulo. Laboratório de Imunologia, Instituto do Coração. Sao Paulo, SP, Brazil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brazil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Investigação em Imunologia-Instituto do Milênio. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil. | pt_BR |
| Subject | Vaccine | pt_BR |
| Subject | Human T cells | pt_BR |
| Subject | Synthetic peptides | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | Sm14 | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
