| Author | Garcia, Luiz Fernando Cardoso | |
| Author | Wowk, Pryscilla Fanini | |
| Author | Albrecht, Letusa | |
| Access date | 2024-12-11T01:22:52Z | |
| Available date | 2024-12-11T01:22:52Z | |
| Document date | 2024 | |
| Citation | GARCIA, L. F. C. et al. Unraveling the impact of extracellular vesicle-depleted serum on endothelial cell characteristics over time. International Journal of Molecular Sciences, v. 25, n. 4761, p. 1-30, 2024. | en_US |
| ISSN | 1422-0067 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/67527 | |
| Description | The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/ijms25094761/s1. | en_US |
| Sponsorship | This project received financial support from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPQ) (Grant 442377/2019-3), and PEP-ICC-FIOCRUZ (Grant ICC-008-FIO-21-2-15). | en_US |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Vesículas extracelulares | en_US |
| Subject in Portuguese | Células endoteliais | en_US |
| Subject in Portuguese | Soro fetal bovino | en_US |
| Subject in Portuguese | Cultura de célula | en_US |
| Subject in Portuguese | Proteômica | en_US |
| Title | Unraveling the impact of extracellular vesicle-depleted serum on endothelial cell characteristics over time | en_US |
| Type | Article | en_US |
| DOI | 10.3390/ijms25094761 | |
| Abstract | Extracellular vesicles (EVs) are produced by all kinds of cells, including endothelial cells. It has been observed that EVs present in fetal bovine serum (FBS), broadly used in cell culture, can be a confounding factor and lead to misinterpretation of results. To investigate this phenomenon, human brain microvascular endothelial cells (HBMECs) were cultured for 2 or 24 h in the presence of EVdepleted FBS (EVdS). Cell death, gene and protein expression, and the presence of EVs isolated from these cells were evaluated. The uptake of EVs, intercellular adhesion molecule 1 (ICAM-1) expression, and monocyte adhesion to endothelial cells exposed to EVs were also evaluated. Our results revealed higher apoptosis rates in cells cultured with EVdS for 2 and 24 h. There was an increase in interleukin 8 (IL8) expression after 2 h and a decrease in interleukin 6 (IL6) and IL8 expression after 24 h of culture. Among the proteins identified in EVs isolated from cells cultured for 2 h (EV2h), several were related to ribosomes and carbon metabolism. EVs from cells cultured for 24 h (EV24h) presented a protein profile associated with cell adhesion and platelet activation. Additionally, HBMECs exhibited increased uptake of EV2h. Treatment of endothelial cells with EV2h resulted in greater ICAM-1 expression and greater adherence to monocytes than did treatment with EV24h. According to our data, HBMEC cultivated with EVdS produce EVs with different physical characteristics and protein levels that vary over time. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Pesquisa em Apicomplexa. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. | en_US |
| Subject | Extracellular vesicles | en_US |
| Subject | Endothelial cells | en_US |
| Subject | Fetal bovine serum | en_US |
| Subject | Cell culture | en_US |
| Subject | Proteomics | en_US |
| DeCS | Vesículas extracelulares | en_US |
| DeCS | Técnicas de Cultura de Células | en_US |
| DeCS | Células Endoteliais | en_US |
