| Author | Torres, Ana Cláudia Martins Braga Gomes | |
| Author | Mathias, Carolina | |
| Author | Baal, Suelen Cristina Soares | |
| Author | Kohler, Ana Flávia | |
| Author | Cunha, Mylena Lemes | |
| Author | Blanes, Lucas | |
| Access date | 2024-12-16T16:22:31Z | |
| Available date | 2024-12-16T16:22:31Z | |
| Document date | 2024 | |
| Citation | TORRES, A. C. M. B. G. et al. Advancements in LAMP-based diagnostics: emerging techniques and applications in viral detection with a focus on herpesviruses in transplant patient management. Internacional Journal of Molecular Sciences, v. 25, n. 11506, p. 1-31, 2024. | en_US |
| ISSN | 1422-0067 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/67620 | |
| Sponsorship | This research was funded by Fiocruz grant number VPPIS-004-FIO-22-2-4 and CNPq (National Council for Scientific and Technological Development) research grant 440387/2022-1. | en_US |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Beira de leito | en_US |
| Subject in Portuguese | Detecção viral | en_US |
| Subject in Portuguese | Amplificação isotermal | en_US |
| Title | Advancements in LAMP-based diagnostics: emerging techniques and applications in viral detection with a focus on herpesviruses in transplant patient management | en_US |
| Type | Article | en_US |
| DOI | 10.3390/ijms252111506 | |
| Abstract | Loop-mediated isothermal amplification (LAMP) is a highly effective molecular diagnostic technique, particularly advantageous for point-of-care (POC) settings. In recent years, LAMP has expanded to include various adaptations such as DARQ-LAMP, QUASR, FLOS-LAMP, displacement probes and molecular beacons. These methods enable multiplex detection of multiple targets in a single reaction, enhancing cost-effectiveness and diagnostic efficiency. Consequently, LAMP has gained significant traction in diagnosing diverse viruses, notably during the COVID-19 pandemic. However, its application for detecting Herpesviridae remains relatively unexplored. This group of viruses is of particular interest due to their latency and potential reactivation, crucial for immunocompromised patients, including organ and hematopoietic stem cell transplant recipients. This review highlights recent advancements in LAMP for virus diagnosis and explores current research trends and future prospects, emphasizing the detection challenges posed by Herpesviridae. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | en_US |
| Affilliation | Federal University of Parana. Department of Genetics. Post-Graduation Program in Genetics. Curitiba, PR, Brasil. | en_US |
| Affilliation | Federal University of Parana. Department of Genetics. Post-Graduation Program in Genetics. Curitiba, PR, Brasil. | en_US |
| Affilliation | Federal University of Parana. Department of Genetics. Post-Graduation Program in Genetics. Curitiba, PR, Brasil. | en_US |
| Affilliation | Federal University of Parana. Department of Genetics. Post-Graduation Program in Genetics. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | en_US |
| Subject | Point-of-care | en_US |
| Subject | LAMP | en_US |
| Subject | Viral detection | en_US |
| Subject | Herpesviridae | en_US |
| Subject | Isothermal amplification | en_US |
| DeCS | Infecções por Herpesviridae | en_US |
| DeCS | Testes Imediatos | en_US |
