| Author | Carmo, Paulo Henrique Fonseca Do | |
| Author | Lage, Anna Carolina Pinheiro | |
| Author | Garcia, Maíra Terra | |
| Author | Silva, Newton Soares da | |
| Author | Santos, Daniel Assis | |
| Author | Mylonakis, Eleftherios | |
| Author | Junqueira, Juliana Campos | |
| Access date | 2024-12-16T16:50:01Z | |
| Available date | 2024-12-16T16:50:01Z | |
| Document date | 2024 | |
| Citation | DO CARMO, Paulo Henrique Fonseca et al. Resveratrol-coated gold nanorods produced by green synthesis with activity against Candida albicans. Virulence, v. 15, n. 1, 2416550, 2024. doi: 10.1080/21505594.2024.2416550 | en_US |
| ISSN | 2150-5594 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/67623 | |
| Language | eng | en_US |
| Publisher | Taylor & Francis | en_US |
| Rights | restricted access | |
| Title | Resveratrol-coated gold nanorods produced by green synthesis with activity against Candida albicans. | en_US |
| Type | Article | |
| Abstract | Candida albicans is an opportunistic yeast capable of causing a wide range of mucosal, cutaneous, and systemic infections. However, therapeutic strategies are limited to a few antifungal agents. Inorganic nanoparticles have been investigated as carrier systems for antifungals as potential new treatments. In this study, we focused on the antifungal activity of gold nanorods, a specific rod-shaped gold nanoparticle, produced by green synthesis using resveratrol as a metal-reducing agent. The synthesis method resulted in stable control nanoparticles (AuNp) and resveratrol-coated gold nanoparticles (AuNpRSV) with medium sizes of 32.4 × 15.9 nm for AuNp, and 33.5 × 15.3 nm for AuNpRSV. Both AuNp and AuNpRSV inhibited the C. albicans grown at 2.46 µg/mL, exhibited fungicidal effects at 4.92 µg/mL, and significantly decreased filamentation, biofilm viability, reactive oxygen species production and ergosterol levels of C. albicans. In addition, exposure to AuNpRSV reduced the ability of C. albicans to grow in the presence of cell membrane stressors. Transmission electron microscopy revealed enlargement of the cell wall and retraction of the cell membrane after treatment with AuNp and AuNpRSV. Promisingly, in vivo toxicity analysis demonstrated that both nanoparticles maintained the full viability of Galleria mellonella larvae at 49.20 µg/mL. In conclusion, both gold nanoparticles exhibited antifungal activity; however, these effects were enhanced by AuNpRSV. Altogether, AuNps and AuNpRSVs are potential antifungal agents for the treatment of C. albicans infections. | en_US |
| Affilliation | Department of Biosciences and Oral Diagnosis. Institute of Science and Technology. São Paulo State University. São José dos Campos, SP, Brazil | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Departamento de Medicina. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Department of Biosciences and Oral Diagnosis. Institute of Science and Technology. São Paulo State University. São José dos Campos, SP, Brazil | en_US |
| Affilliation | Department of Biosciences and Oral Diagnosis. Institute of Science and Technology. São Paulo State University. São José dos Campos, SP, Brazil | en_US |
| Affilliation | Department of Microbiology. Institute of Biological Sciences. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
| Affilliation | Department of Medicine. Houston Methodist Hospital. Houston, TX, USA | en_US |
| Affilliation | Department of Biosciences and Oral Diagnosis. Institute of Science and Technology. São Paulo State University. São José dos Campos, SP, Brazil | en_US |
| Subject | Gold nanoparticles | en_US |
| Subject | Biofilm | en_US |
| Subject | Ergosterol | en_US |
| Subject | Filamentation | en_US |
| Subject | Reactive oxygen species | en_US |
| Embargo date | 3100-12-31 | |
