| Author | Kushwaha, Simran Krishnakant | |
| Author | Wu, Yi | |
| Author | Avila, Hugo Leonardo | |
| Author | Anand, Abhirath | |
| Author | Sicheritz-Pontén, Thomas | |
| Author | Millard, Andrew | |
| Author | Marathe, Sandhya Amol | |
| Author | Nobrega, Franklin L | |
| Access date | 2024-12-17T10:02:22Z | |
| Available date | 2024-12-17T10:02:22Z | |
| Document date | 2024 | |
| Citation | KUSHWAHA, S. K. et al. Comprehensive blueprint of Salmonella genomic plasticity identifies hotspots for pathogenicity genes. PLoS Biology, v. 22, n. 8 (e3002746), p. 1-24, 2024. | en_US |
| ISSN | 1545-7885 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/67640 | |
| Sponsorship | This work was supported by British Council Newton Bhabha Fund [grant number 654669088] to S.K.K and Wessex Medical Trust [grant number AB03] to F.L.N. | en_US |
| Language | eng | en_US |
| Publisher | PLOS | en_US |
| Rights | open access | en_US |
| Subject in Portuguese | Salmonella | en_US |
| Subject in Portuguese | Infecção bacteriana | en_US |
| Subject in Portuguese | Regiões de plasticidade genômica | en_US |
| Title | Comprehensive blueprint of Salmonella genomic plasticity identifies hotspots for pathogenicity genes | en_US |
| Type | Article | en_US |
| DOI | 10.1371/journal.pbio.3002746 | |
| Abstract | UUnderstanding the dynamic evolution of Salmonella is vital for effective bacterial infection management. This study explores the role of the flexible genome, organised in regions of genomic plasticity (RGP), in shaping the pathogenicity of Salmonella lineages. Through comprehensive genomic analysis of 12,244 Salmonella spp. genomes covering 2 species, 6 ubspecies, and 46 serovars, we uncover distinct integration patterns of pathogenicity-related gene clusters into RGP, challenging traditional views of gene distribution. These RGP exhibit distinct preferences for specific genomic spots, and the presence or absence of
such spots across Salmonella lineages profoundly shapes strain pathogenicity. RGP preferences are guided by conserved flanking genes surrounding integration spots, implicating their involvement in regulatory networks and functional synergies with integrated gene clusters. Additionally, we emphasise the multifaceted contributions of plasmids and prophages to the pathogenicity of diverse Salmonella lineages. Overall, this study provides a comprehensive blueprint of the pathogenicity potential of Salmonella. This unique insight identifies genomic spots in nonpathogenic lineages that hold the potential for harbouring pathogenicity genes, providing a foundation for predicting future adaptations and developing targeted strategies against emerging human pathogenic strains. | en_US |
| Affilliation | Birla Institute of Technology & Science. Department of Biological Sciences. Pilani, Rajasthan, India / University of Southampton. School of Biological Sciences. Southampton, United Kingdom. | en_US |
| Affilliation | University of Southampton. School of Biological Sciences. Southampton, United Kingdom. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | en_US |
| Affilliation | Birla Institute of Technology & Science. Department of Computer Sciences and Information Systems. Pilani, Rajasthan, India. | en_US |
| Affilliation | University of Copenhagen. Globe Institute. Center for Evolutionary Hologenomics. Copenhagen, Denmark / AIMST University. Centre of Excellence for Omics-Driven Computational Biodiscovery. Bedong, Kedah, Malaysia. | en_US |
| Affilliation | University of Leicester. Centre for Phage Research. Department of Genetics and Genome Biology. Leicester, United Kingdom. | en_US |
| Affilliation | Birla Institute of Technology & Science. Department of Biological Sciences. Pilani, Rajasthan, India. | en_US |
| Affilliation | University of Southampton. School of Biological Sciences. Southampton, United Kingdom. | en_US |
| Subject | Regions of genomic plasticity | en_US |
| Subject | Salmonella | en_US |
| Subject | Bacterial infection | en_US |
