| Author | Cerri, Fabiano | |
| Author | Araújo, Maisa da Silva | |
| Author | Aguirre, André de Abreu Rangel | |
| Author | Evaristo, Geisa Paulino Caprini | |
| Author | Evaristo, Joseph Albert Medeiros | |
| Author | Nogueira, Fábio César Sousa | |
| Author | Medeiros, Jansen Fernandes de | |
| Author | Dias, Quintino Moura | |
| Access date | 2024-12-20T16:11:51Z | |
| Available date | 2024-12-20T16:11:51Z | |
| Document date | 2023 | |
| Citation | CERRI, Fabiano et al. Crude saliva of Amblyomma cajennense sensu stricto (Acari: Ixodidae) reduces locomotor activity and increases the hemocyte number in the females of Aedes aegypti (Diptera: Culicidae). Experimental Parasitology, v. 251, n. 108570. 2023. | en_US |
| ISSN | 0014-4894 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/67736 | |
| Sponsorship | CNPq/FAPERO | en_US |
| Language | eng | en_US |
| Publisher | Elsevier | en_US |
| Rights | open access | en_US |
| Title | Crude saliva of Amblyomma cajennense sensu stricto (Acari: Ixodidae) reduces locomotor activity and increases the hemocyte number in the females of Aedes aegypti (Diptera: Culicidae) | en_US |
| Type | Article | en_US |
| DOI | 10.1016/j.exppara.2023.108570 | |
| Abstract | Aedes aegypti are vector insects of arboviruses such as dengue, Zika, and chikungunya. All available vector
control methods have limited efficacy, highlighting the urgent need to find alternative ones. Evidence shows that arachnids like ticks are sources of biologically active compounds. Moreover, chemical modulation of the locomotor and immune systems of vector insects can be used to control arbovirus transmission. The present study evaluated the effectiveness of crude saliva of female Amblyomma cajennense sensu stricto (s.s.) ticks in reducing locomotor activity and inducing an immune response in Ae. aegypti females. Additionally, the study evaluated the protein constitution of tick saliva. For this purpose, the crude saliva obtained from several semi-engorged A. cajennense females was used. A volume of 0.2 nL of crude tick saliva was administered to mosquitoes by direct intrathoracic microinjection. The effect of the tick’s saliva on the locomotor activity of the mosquito was observed using Flybox, a video-automated monitoring system, and the hemolymph hemocyte levels were quantified by reading slides under a light microscope. The protein concentration of the crude tick saliva was 1.27 μg/μL, and its electrophoretic profile indicates the presence of proteins with a molecular weight ranging between -7 and 95 kDa. Microplusins, ixodegrins, cystatin, actins, beta-actin, calponin, albumin, alpha-globulins, and hemoglobin were the main proteins identified by proteomics in the saliva of A. cajennense. The microinjected saliva had low toxicity for Ae. aegypti females and significantly reduced their locomotor activity, especially in the
transition between the light and dark phases. The crude tick saliva did not change the period and rhythmicity of the circadian cycle. The tick saliva significantly increased the number of hemocytes two days after injection and reduced it after five days. These results suggest that further evaluation of the biological properties of tick saliva proteins against Ae. aegypti would be of interest. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de Neuro e Imunofarmacologia. Porto Velho, RO, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de pós-graduação em Biologia Experimental. Porto Velho, RO, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de entomologia. Plataforma de Produção e Infecção de Vetores da Malária. Porto Velho, RO, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Fiocruz Rondônia. Plataforma de Criação e Experimentação Animal. Porto Velho, RO, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Fiocruz Rondônia. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Porto Velho, RO, Brasil. | en_US |
| Affilliation | Centro Universitário São Lucas. Porto Velho, RO, Brasil. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Laboratório de Proteômica. Rio de Janeiro, RJ, Brasil | en_US |
| Subject | Aedes aegypti locomotor Activity | en_US |
| Subject | Amblyomma cajennense crude Saliva | en_US |
| Subject | Hemocytes | en_US |
| Subject | Proteomic analysis | en_US |
| DeCS | Aedes aegypti | en_US |
| DeCS | Amblyomma | en_US |
| DeCS | Hemocytes | en_US |
