| Author | Mazepa, Ester | |
| Author | Cunha, Elizabeth Sousa | |
| Author | Valerio, Hellen Paula | |
| Author | Di Mascio, Paolo | |
| Author | Batista, Michel | |
| Author | Marchini, Fabricio Klerynton | |
| Author | Meira, Willian Vanderlei | |
| Author | Noleto, Guilhermina Rodrigues | |
| Author | Winnischofer, Sheila Maria Brochado | |
| Author | Martinez, Glaucia Regina | |
| Access date | 2024-12-23T18:27:03Z | |
| Available date | 2024-12-23T18:27:03Z | |
| Document date | 2024 | |
| Citation | MAZEPA, Ester et al. Unveiling novel targets in melanoma under melanogenesis stimulation and photodynamic therapy by redox proteomics. Photochemistry and Photobiology, v. 00, p. 1-16, 2024. | en_US |
| ISSN | 1751-1097 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/67764 | |
| Sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Grant/Award Number: 88887372486/2019-00; Conselho Nacional de Desenvolvimento Científico e Tecnológico, Grant/Award Number: 311259/2021-2 and 422547/2018-3. | en_US |
| Language | eng | en_US |
| Publisher | WILEY | en_US |
| Rights | restricted access | |
| Subject in Portuguese | Proteômica | en_US |
| Subject in Portuguese | Melanina | en_US |
| Subject in Portuguese | Melanogênese | en_US |
| Subject in Portuguese | Terapia fotodinâmica | en_US |
| Subject in Portuguese | Rosa Bengala | en_US |
| Title | Unveiling novel targets in melanoma under melanogenesis stimulation and photodynamic therapy by redox proteomics | en_US |
| Type | Article | |
| DOI | 10.1111/php.13994 | |
| Abstract | Melanogenesis-stimulated B16-F10 cells enter in a quiescent state, present inhibited mitochondrial respiration and increased reactive oxygen species levels. These alterations suggest that these cells may be under redox signaling, allowing tumor survival. The aim of this study was to evaluate redox-modified proteins in B16-F10 cells after melanogenesis stimulation and rose bengal-photodynamic therapy (RB-PDT). A redox proteomics label-free approach based on the biotin switch assay technique with biotin-HPDP and N-ethylmaleimide was used to assess the thiol-oxidized protein profile. Aconitase was oxidized at Cys-448 and Cys-451, citrate synthase was oxidized at Cys-202 and aspartate aminotransferase (Got2) was oxidized at Cys-272 and Cys-274, exclusively after melanogenesis stimulation. After RB-PDT, only guanine nucleotide-binding protein subunit beta-2-like 1 (Gnb2l1) was oxidized (Cys-168). In contrast, melanogenesis stimulation followed by RB-PDT led to the oxidation of different cysteines in Gnb2l1 (Cys-153 and Cys-249). Besides that, glyceraldehyde-3-phosphate dehydrogenase (Gapdh) presented oxidation at Cys-245, peptidyl-prolyl cis-trans isomerase A (Ppia) was oxidized at Cys-161 and 5,6-dihydroxyindole-2-carboxylic acid oxidase (Tyrp1) was oxidized at Cys-65, Cys-30, and Cys-336 after melanogenesis stimulation followed by RB-PDT. The redox alterations observed in murine melanoma cells and identification of possible target proteins are of great importance to further understand tumor resistance mechanisms. | en_US |
| Affilliation | Federal University of Paraná. Department of Biochemistry and Molecular Biology. Postgraduate Program in Biochemistry Sciences. Curitiba, PR, Brasil. | en_US |
| Affilliation | Federal University of Paraná. Department of Biochemistry and Molecular Biology. Postgraduate Program in Biochemistry Sciences. Curitiba, PR, Brasil. | en_US |
| Affilliation | Instituto Butantan. Centre of Excellence in New Target Discovery. São Paulo, SP, Brasil. | en_US |
| Affilliation | Chemistry Institute. Biochemistry Department. São Paulo, SP, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | en_US |
| Affilliation | Federal University of Paraná. Department of Biochemistry and Molecular Biology. Postgraduate Program in Biochemistry Sciences. Curitiba, PR, Brasil. | en_US |
| Affilliation | Federal University of Paraná. Department of Biochemistry and Molecular Biology. Postgraduate Program in Biochemistry Sciences. Curitiba, PR, Brasil. | en_US |
| Affilliation | Federal University of Paraná. Department of Biochemistry and Molecular Biology. Postgraduate Program in Biochemistry Sciences. Curitiba, PR, Brasil. | en_US |
| Affilliation | Federal University of Paraná. Department of Biochemistry and Molecular Biology. Postgraduate Program in Biochemistry Sciences. Curitiba, PR, Brasil. | en_US |
| Subject | Melanin | en_US |
| Subject | Melanogenesis | en_US |
| Subject | Melanoma | en_US |
| Subject | Photodynamic therapy | en_US |
| Subject | Proteomics | en_US |
| Subject | Redox | en_US |
| Subject | Rose bengal | en_US |
| DeCS | Rosa Bengala | en_US |
| DeCS | Proteômica | en_US |
| DeCS | Melanoma | en_US |
| DeCS | Melanogênese | en_US |
| DeCS | Fotoquimioterapia | en_US |
| Embargo date | 2034 | |
