| Author | Santos, Najara Akira Costa dos | |
| Author | Carvalho, Vanessa Rafaela de | |
| Author | Souza-Neto, Jayme A. | |
| Author | Alonso, Diego Peres | |
| Author | Ribolla, Paulo Eduardo Martins | |
| Author | Medeiros, Jansen Fernandes de | |
| Author | Araujo, Maisa da Silva | |
| Access date | 2024-12-26T14:57:36Z | |
| Available date | 2024-12-26T14:57:36Z | |
| Document date | 2023 | |
| Citation | SANTOS, Najara Akira Costa dos et al. Bacterial Microbiota from Lab-Reared and Field-Captured Anopheles darlingi Midgut and Salivary Gland. 5. ed. Microorganisms, v. 11, 2023. | en_US |
| ISSN | 2076-2607 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/67784 | |
| Sponsorship | Ministério da Saúde/DECIT/CNPq
Fundação Bill & Melinda Gates
PPSUS/Fundação de Amparo à Pesquisa Estado de Rondônia (FAPERO)
Fundação de Amparo à Pesquisa do Estado de São Paulo-FAPESP
CNPq | en_US |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | en_US |
| Title | Bacterial Microbiota from Lab-Reared and Field-Captured Anopheles darlingi Midgut and Salivary Gland | en_US |
| Type | Article | en_US |
| DOI | 10.3390/microorganisms11051145 | |
| Abstract | Anopheles darlingi is a major malaria vector in the Amazon region and, like other vectors, harbors a community of microorganisms with which it shares a network of interactions. Here, we describe the diversity and bacterial composition from the midguts and salivary glands of lab-reared and field-captured An. darlingi using metagenome sequencing of the 16S rRNA gene. The libraries were built using the amplification of the region V3–V4 16S rRNA gene. The bacterial community from the salivary glands was more diverse and richer than the community from the midguts. However, the salivary glands and midguts only showed dissimilarities in beta diversity between lab-reared mosquitoes. Despite that, intra-variability was observed in the samples. Acinetobacter and Pseudomonas were dominant in the tissues of lab-reared mosquitoes. Sequences of Wolbachia and Asaia were both found in the tissue of lab-reared mosquitoes; however, only Asaia was found in field-captured An. darlingi, but in low abundance. This is the first report on the characterization of microbiota composition from the salivary glands of An. darlingi from lab-reared and field-captured individuals. This study can provide invaluable insights for future investigations regarding mosquito development and interaction between mosquito microbiota and Plasmodium sp. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de Entomologia. Plataforma de Produção e Infecção de Vetores da Malária. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de Pós-Graduação em Biologia Experimental. Porto Velho, RO, Brasil. | en_US |
| Affilliation | São Paulo State University. School of Agricultural Sciences. Department of Bioprocesses and Biotechnology. Multiuser Central Laboratory. Botucatu, SP, Brasil. | en_US |
| Affilliation | São Paulo State University. School of Agricultural Sciences. Department of Bioprocesses and Biotechnology. Multiuser Central Laboratory. Botucatu, SP, Brasil. | en_US |
| Affilliation | Universidade Estadual Paulista. Instituto de Biotecnologia da UNESP. Departamento de Biotecnologia. Botucatu, SP, Brasil. | en_US |
| Affilliation | Universidade Estadual Paulista. Instituto de Biotecnologia da UNESP. Departamento de Biotecnologia. Botucatu, SP, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de Entomologia. Plataforma de Produção e Infecção de Vetores da Malária. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de Pós-Graduação em Biologia Experimental. Porto Velho, RO, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de Entomologia. Plataforma de Produção e Infecção de Vetores da Malária. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Departamento de Biologia. Programa de Pós-Graduação em Conservação e uso de Recursos Naturais. Porto Velho, RO, Brasil / Centro de Pesquisa em Medicina Tropical. Laboratório de Pesquisa Translacional e Clínica. Porto Velho, RO, Brasil. | en_US |
| Subject | 16S rRNA | en_US |
| Subject | Amazon region | en_US |
| Subject | Bacteria | en_US |
| Subject | Malaria vector | en_US |
| Subject | Microbiota | en_US |
| Subject | Midgut | en_US |
| Subject | Salivary gland | en_US |
| DeCS | RNA, Ribosomal, 16S | en_US |
| DeCS | Bacteria | en_US |
| DeCS | Malaria | en_US |
| DeCS | Mycobiome | en_US |
