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https://www.arca.fiocruz.br/handle/icict/6779
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ArtigoDireito Autoral
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- IOC - Artigos de Periódicos [12793]
- MG - IRR - Artigos de Periódicos [4172]
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APPLICATION OF DIRECT AGGLUTINATION TEST (DAT) AND FAST AGGLUTINATION SCREENING TEST (FAST) FOR SERO-DIAGNOSIS OF VISCERAL LEISHMANIASIS IN ENDEMIC AREA OF MINAS GERAIS, BRAZIL
Elisa
PCR
Direct Agglutination Test
Fast Agglutination Screening Test
Autor(es)
Afiliação
Universidade do Estado de Minas Gerais. Belo Horizonte, MG, Brasil / Fundação Educacional de Divinópolis. Divinópolis, MG, Brasil.
Koninklijk Instituut voor de Tropen / Royal Tropical Institute. Biomedical Research. Amsterdam, The Netherlands.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Koninklijk Instituut voor de Tropen / Royal Tropical Institute. Biomedical Research. Amsterdam, The Netherlands.
Koninklijk Instituut voor de Tropen / Royal Tropical Institute. Biomedical Research. Amsterdam, The Netherlands.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Koninklijk Instituut voor de Tropen / Royal Tropical Institute. Biomedical Research. Amsterdam, The Netherlands.
Resumo em Inglês
Background: The direct agglutination test (DAT) has proved to be a very important sero-diagnostic tool combining high levels of intrinsic validity and ease of performance. Otherwise, fast agglutination screening test (FAST) utilises only one serum dilution making the test very suitable for the screening of large populations.
Results: We have tested FAST and DAT for the detection anti-Leishmania antibodies in serum samples from patients with American visceral (AVL) and cutaneous leishmaniases (ACL) in Minas Gerais State, Brazil. The DAT on serum and blood samples of confirmed AVL patients found all samples positive at a serum dilution of ≥1:800. This dilution was subsequently used as cut off value in the study. The blood and serum samples of these confirmed patients could also be clearly read in FAST using a 1:100 dilution with the same high sensitivity. DAT and FAST were not able to detect significant amounts of antibodies in samples from ACL patients and are not suitable for the diagnosis of this manifestation of the disease.
Conclusion: We suggest that both DAT and FAST are very practical diagnostic tools for the sero-diagnosis of AVL under rural conditions as bothserological tests do not require sophisticated equipment, a cold chain and are very simple to perform.
Palavras-chave em inglês
Sero-diagnosisElisa
PCR
Direct Agglutination Test
Fast Agglutination Screening Test
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