| Author | Mascarenhas, Maria Eduarda Pereira | |
| Author | Louzada, Jaime | |
| Author | Rosa, Renato Amorim | |
| Author | Assis, Gabriela Maíra Pereira de | |
| Author | Kano, Flora Satiko | |
| Author | Ferreira, Joseli de Oliveira | |
| Author | Sousa, Taís Nóbrega de | |
| Access date | 2024-12-26T17:28:12Z | |
| Available date | 2024-12-26T17:28:12Z | |
| Document date | 2024 | |
| Citation | MASCARENHAS, Maria Eduarda Pereira et al. Malaria rapid diagnostic tests: very low prevalence of plasmodium falciparum histidine-rich protein 2 (pfhrp2) deletion in the Brazil-Venezuela-Guyana tri-border. Research Square, p. 1-15, 26 July 2024. | |
| ISSN | 2693-5015 | |
| URI | https://www.arca.fiocruz.br/handle/icict/67803 | |
| Description | Produção científica do Laboratório de Imunoparasitologia. | pt_BR |
| Sponsorship | This work was supported by the Minas Gerais State Research Support Foundation (FAPEMIG), the National Council for Scientic and Technological Development (CNPq), PAEF/ FIOCRUZ (IOC-008-FIO-22-2-49), and Inova Fiocruz Innovative Ideas Program. Taís Nóbrega de Sousa and Joseli de Oliveira Ferreira are recipients of a CNPq Research Productivity fellowship. | |
| Language | eng | en_US |
| Later version | https://www.arca.fiocruz.br/handle/icict/67877 | |
| Rights | open access | |
| Title | Malaria rapid diagnostic tests: very low prevalence of plasmodium falciparum histidine-rich protein 2 (pfhrp2) deletion in the Brazil-Venezuela-Guyana tri-border | en_US |
| Type | Preprint | |
| DOI | 10.21203/rs.3.rs-4641495/v1 | |
| Abstract | Rapid Diagnostic Tests (RDTs) have been an important diagnostic tool for detecting P. falciparum malaria in resource-limited settings. Most tests are designed to detect the Histidine-rich Protein 2 (HRP2). Parasites lacking pfhrp2 and its homologous pfhrp3 have been reported in several regions, with prevalence reaching 100% in certain areas. To better characterize P. falciparum isolates circulating in the Brazil-Venezuela-Guyana tri-border region, we performed a comprehensive analysis of 365 samples collected between 2016 and 2018. Molecular and immunological methods were employed to detect HRP2 and conrm pfhrp2/3 deletion. Our ndings point to a low prevalence (1%) of pfhrp2-deleted parasites conrmed by the lack of HRP2 detection. Among false-negative HRP2-RDT tests (6%), most were attributed to low parasite densities. A merozoite surface protein 2 (msp2)-based intra-host diversity analysis suggested overall low genetic diversity. The pattern of HRP2 sequences resembled that has been previously described in areas along the Brazil and French Guiana border. In conclusion, we have found a low prevalence of pfhrp2-deleted parasites in the north-central Guiana Shield, which contrasts with the ndings reported at the Peru border. Continued surveys are necessary to monitor the prevalence of pfhrp2 deletion in this area characterized by a high number of cross-border malaria cases. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa de Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brasil. | |
| Affilliation | Universidade Federal de Roraima. Boa Vista, RR, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa de Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa de Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa de Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa de Biologia Molecular e Imunologia da Malária. Belo Horizonte, MG, Brasil / Karolinska Institutet. Department of Microbiology, Tumor and Cell Biology. Solna, Sweden. | |
| Subject | Malaria | en_US |
| Subject | Plasmodium falciparum | en_US |
| Subject | pfhrp2 | en_US |
| Subject | pfhrp3 | en_US |
| Subject | RDT | en_US |
| Subject | Genetic diversity | en_US |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
| xmlui.metadata.dc.subject.ods | 09 Indústria, inovação e infraestrutura | |
| xmlui.metadata.dc.subject.ods | 17 Parcerias e meios de implementação | |
