| Author | Reis, Laise Rodrigues | |
| Author | Silva-Moraes, Vanessa | |
| Author | Carvalho, Andréa Teixeira de | |
| Author | Ross, Ted M | |
| Access date | 2025-01-07T15:55:02Z | |
| Available date | 2025-01-07T15:55:02Z | |
| Document date | 2024 | |
| Citation | REIS, Laise Rodrigues et al. B-cell dynamics underlying poor response upon split-inactivated influenza virus vaccination. Frontiers in Immunology, v. 15, p. 1481910, 2024. | en_US |
| ISSN | 1664-3224 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/67924 | |
| Language | eng | en_US |
| Publisher | Frontiers Research Foundation | en_US |
| Rights | open access | en_US |
| Title | B-cell dynamics underlying poor response upon split-inactivated influenza virus vaccination. | en_US |
| Type | Article | en_US |
| DOI | 10.3389/fimmu.2024.1481910 | |
| Abstract | This investigation elucidated the differences in humoral and H1N1 HA-specific memory B-cells response in participants exhibiting distinct immune response patterns prior to and after vaccination with Fluzone, the quadrivalent split-inactivated seasonal influenza virus vaccine. Participants were categorized into persistent non-responders and persistent responders based on their hemagglutination-inhibition (HAI) antibody titers to the H1N1 component from each vaccine administered between the 2019-2020 to 2023-2024 seasons. Persistent responders had higher fold change in H1N1 HA-specific CD21 expressing B-cells, plasmablasts, and plasma cells. A significant increase in H1N1 HA-specific transitional B-cells in persistent non-responders was observed. The frequency and fold change of H1N1-specific IgM-expressing memory B-cells was higher in persistent non-responders. Dimensionality reduction analysis also demonstrated higher IgM expression for persistent non-responders than persistent responders. Furthermore, persistent non-responders had a significant fold change increase in IgA tissue-like memory, IgG exhausted tissue-like memory, and double negative (DN) activated memory cells. In contrast, persistent responders had increased frequency of IgG-activated memory B-cells, IgG resting B-cells and DN resting B-cells. Correlation analysis revealed a positive correlation between HAI titers and DN memory B-cells and a negative correlation between HAI titers and IgG-expressing memory B-cells in persistent non-responders. Conversely, persistent responders had a positive correlation between HAI titers and IgA resting memory B-cells and a negative correlation between IgG memory B-cells and DN memory B-cells. Overall, this study provided valuable insights into the differential immune memory B-cell responses following influenza virus vaccination and paves the way for future research to further unravel the complexities of vaccine-induced memory B-cells and ultimately improve vaccination strategies against influenza virus infection. | en_US |
| Affilliation | Cleveland Clinic. Florida Research and Innovation Center. Port Saint Lucie, FL, United States. | en_US |
| Affilliation | Cleveland Clinic. Florida Research and Innovation Center. Port Saint Lucie, FL, United States. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou Institute. Grupo integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG Brasil. | en_US |
| Affilliation | Cleveland Clinic. Florida Research and Innovation Center. Port Saint Lucie, FL, United States / Cleveland Clinic. Lerner Research Institute. Department of Infection Biology. Cleveland, OH, United States / University of Georgia. Department of Infectious Diseases. Athens, GA, United States / University of Georgia. Center for Vaccines and Immunology. Athens, GA, United States. | en_US |
| Subject | Fluzone vaccine | en_US |
| Subject | Adults | en_US |
| Subject | Humoral response | en_US |
| Subject | Influenza | en_US |
| Subject | Memory B-cells | en_US |
