| Author | Bansal, Geetha P. | |
| Author | Araujo, Maisa da Silva | |
| Author | Cao, Yi | |
| Author | Shaffer, Emily | |
| Author | Araujo, Jessica Evangelista | |
| Author | Medeiros, Jansen Fernandes | |
| Author | Hayashi, Clifford | |
| Author | Vinetz, Joseph | |
| Author | Kumar, Nirbhay | |
| Access date | 2025-01-13T19:09:55Z | |
| Available date | 2025-01-13T19:09:55Z | |
| Document date | 2024 | |
| Citation | BANSAL, Geetha P. et al. Transmission-reducing and -enhancing monoclonal antibodies against Plasmodium vivax gamete surface protein Pvs48/45. Infection and Immunity, v. 92, n. 3, 2024. | en_US |
| ISSN | 1098-5522 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/68073 | |
| Language | eng | en_US |
| Publisher | American Society for Microbiology | en_US |
| Rights | open access | en_US |
| Title | Transmission-reducing and -enhancing monoclonal antibodies against Plasmodium vivax gamete surface protein Pvs48/45 | en_US |
| Type | Article | en_US |
| DOI | 10.1128/iai.00374-23 | |
| Abstract | Gamete surface protein P48/45 has been shown to be important for male gamete fertility and a strong candidate for the development of a malaria transmission-blocking vaccine (TBV). However, TBV development for Plasmodium vivax homolog Pvs48/45 has been slow because of a number of challenges: availability of conformationally suitable recombinant protein; the lack of an in vivo challenge model; and the inability to produce P. vivax gametocytes in culture to test transmission-blocking activity of antibodies. To support ongoing efforts to develop Pvs48/45 as a potential vaccine candidate, we initiated efforts to develop much needed reagents to move the fieldfieldforward. We generated monoclonal antibodies (mAbs) directed against Pvs48/45 and characterized putative functional domains in Pvs48/45 using recombinant fragments corresponding to domains D1–D3 and their biological functionality through ex vivo direct membrane feeding assays (DMFAs) using P. vivax parasites from patients in a field setting in Brazil. While some mAbs partially blocked oocyst development in the DMFA, one mAb caused a significant enhancement of the infectivity of gametocytes in the mosquitoes. Individual mAbs exhibiting blocking and enhancing activities recognized non-overlapping epitopes in Pvs48/45. Further characterization of precise epitopes recognized by transmission-reducing and -enhancing antibodies will be crucial to design an effective immunogen with optimum transmission-reducing potential. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de Entomologia. Plataforma de Produção e Infecção de Vetores da Malária. Porto Velho, RO, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de pós-graduação em Biologia Experimental. Porto Velho, RO, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Programa de pós-graduação em Biologia Experimental. Porto Velho, RO, Brasil. | en_US |
| Affilliation | Tulane University. Department of Tropical Medicine. New Orleans, Louisiana, USA. | en_US |
| Affilliation | Tulane University. Department of Tropical Medicine. New Orleans, Louisiana, USA. | en_US |
| Affilliation | Tulane University. Department of Tropical Medicine. New Orleans, Louisiana, USA. | en_US |
| Affilliation | George Washington University. Department of Global Health. Washington, DC, USA. | en_US |
| Affilliation | Yale School of Medicine. New Haven, Connecticut, USA. | en_US |
| Affilliation | Tulane University. Department of Tropical Medicine. New Orleans, Louisiana, USA / George Washington University. Department of Global Health. Washington, DC, USA. | en_US |
| Subject | Plasmodium vivax | en_US |
| Subject | Pvs48/45 | en_US |
| Subject | Monoclonal antibodies | en_US |
| Subject | Transmission-reducing activity | en_US |
| Subject | Transmission-blocking vaccine | en_US |
| Subject | Transmission-enhancing activity | en_US |
| Subject | Anopheles darlingi | en_US |
| DeCS | Plasmodium vivax, | en_US |
| DeCS | Antibodies, Monoclonal | en_US |
| DeCS | Anopheles | en_US |
