| Author | Cumming, Bridgette M. | |
| Author | Addicott, Kelvin W. | |
| Author | Maruri, Fernanda | |
| Author | Pillay, Vanessa | |
| Author | Asmal, Rukaya | |
| Author | Moodley, Sashen | |
| Author | Durate, Beatriz Barreto | |
| Author | Pereira, Mariana Araújo | |
| Author | Mazibuko, Matilda | |
| Author | Mhlane, Zoey | |
| Author | Mbatha, Nikiwe | |
| Author | Khan, Khadija | |
| Author | Makhari, Senamile | |
| Author | Karim, Farina | |
| Author | Peetluk, Lauren | |
| Author | Pym, Alexander S. | |
| Author | Moosa, Mahomed Yunus S. | |
| Author | Heijden, Yuri F. van der | |
| Author | Sterling, Timothy S. | |
| Author | Andrade, Bruno B. | |
| Author | Leslie, Alasdair | |
| Author | Steyn, Adrie J. C. | |
| Access date | 2025-01-21T16:42:05Z | |
| Available date | 2025-01-21T16:42:05Z | |
| Document date | 2024 | |
| Citation | CUMMING, Bridgette M. et al. Longitudinal mitochondrial bioenergetic signatures of blood monocytes and lymphocytes improve during treatment of drug-susceptible pulmonary tuberculosis patients monocyte/lymphocyte bioenergetic signatures post-TB treatment. Frontiers in Immunology, v. 15, p. 1-14, 2024. | en_US |
| ISSN | 1664-3224 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/68164 | |
| Sponsorship | National Institutes of Health.
Fundação Oswaldo Cruz, Programa de Pesquisa Intramural.
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
National Institute of Allergy and Infectious Diseases (NIAID).
Wellcome Strategic Core. | en_US |
| Language | eng | en_US |
| Publisher | Frontiers Media | en_US |
| Rights | open access | en_US |
| Title | Longitudinal mitochondrial bioenergetic signatures of blood monocytes and lymphocytes improve during treatment of drug-susceptible pulmonary tuberculosis patients Monocyte/lymphocyte bioenergetic signatures post-TB treatment | en_US |
| Type | Article | en_US |
| DOI | 10.3389/fimmu.2024.1465448 | |
| Abstract | The impact of human pulmonary tuberculosis (TB) on the bioenergetic metabolism of circulating immune cells remains elusive, as does the resolution of these effects with TB treatment. In this study, the rates of oxidative phosphorylation (OXPHOS) and glycolysis in circulating lymphocytes and monocytes of patients with drug-susceptible TB at diagnosis, 2 months, and 6 months during treatment, and 12 months after diagnosis were investigated using extracellular flux analysis. At diagnosis, the bioenergetic parameters of both blood lymphocytes and monocytes of TB patients were severely impaired in comparison to non-TB and non-HIV-infected controls. However, most bioenergetic parameters were not affected by HIV status or glycemic index. Treatment of TB patients restored the % spare respiratory capacity (%SRC) of the circulating lymphocytes to that observed in non-TB and non-HIV infected controls by 12 months. Treatment also improved the maximal respiration of circulating lymphocytes and the %SRC of circulating monocytes of the TB patients. Notably, the differential correlation of the clinical and bioenergetic parameters of the monocytes and lymphocytes from the controls and TB patients at baseline and month 12 was consistent with improved metabolic health and resolution of inflammation following successful TB treatment. Network analysis of the bioenergetic parameters of circulating immune cells with serum cytokine levels indicated a highly coordinated immune response at month 6. These findings underscore the importance of metabolic health in combating TB, supporting the need for further investigation of the bioenergetic immunometabolism associated with TB infection for novel therapeutic approaches aimed at bolstering cellular energetics to enhance immune responses and expedite recovery in TB patients. | en_US |
| Affilliation | Africa Health Research Institute. University of KwaZulu-Natal. Durban, South Africa. | en_US |
| Affilliation | Africa Health Research Institute. University of KwaZulu-Natal. Durban, South Africa. | en_US |
| Affilliation | Africa Health Research Institute. University of KwaZulu-Natal. Durban, South Africa / Vanderbilt Tuberculosis Center. Vanderbilt University School of Medicine. Nashville, TN, United States. | en_US |
| Affilliation | Africa Health Research Institute. University of KwaZulu-Natal. Durban, South Africa. | en_US |
| Affilliation | Africa Health Research Institute. University of KwaZulu-Natal. Durban, South Africa. | en_US |
| Affilliation | Africa Health Research Institute. University of KwaZulu-Natal. Durban, South Africa. | en_US |
| Affilliation | Multinational Organization Network Sponsoring Translational and Epidemiological Research (MONSTER) Initiative. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Pesquisa Clínica e Translacional. Salvador, BA, Brasil. | en_US |
| Affilliation | Multinational Organization Network Sponsoring Translational and Epidemiological Research (MONSTER) Initiative. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Pesquisa Clínica e Translacional. Salvador, BA, Brasil. | en_US |
| Affilliation | Africa Health Research Institute. University of KwaZulu-Natal. Durban, South Africa. | en_US |
| Affilliation | Africa Health Research Institute. University of KwaZulu-Natal. Durban, South Africa. | en_US |
| Affilliation | Africa Health Research Institute. University of KwaZulu-Natal. Durban, South Africa. | en_US |
| Affilliation | Africa Health Research Institute. University of KwaZulu-Natal. Durban, South Africa. | en_US |
| Affilliation | Africa Health Research Institute. University of KwaZulu-Natal. Durban, South Africa. | en_US |
| Affilliation | Africa Health Research Institute. University of KwaZulu-Natal. Durban, South Africa. | en_US |
| Affilliation | Vanderbilt Tuberculosis Center. Vanderbilt University School of Medicine. Nashville, TN, United States. | en_US |
| Affilliation | Africa Health Research Institute. University of KwaZulu-Natal. Durban, South Africa. | en_US |
| Affilliation | Department of Infectious Diseases. University of KwaZulu-Natal. Durban, South Africa. | en_US |
| Affilliation | Vanderbilt Tuberculosis Center. Vanderbilt University School of Medicine. Nashville, TN, United States / Division of Infectious Diseases. Department of Medicine. Vanderbilt University School of Medicine. Nashville, TN, United States / Global Division. The Aurum Institute. Johannesburg, South Africa. | en_US |
| Affilliation | Vanderbilt Tuberculosis Center. Vanderbilt University School of Medicine. Nashville, TN, United States / Division of Infectious Diseases. Department of Medicine. Vanderbilt University School of Medicine. Nashville, TN, United States. | en_US |
| Affilliation | Multinational Organization Network Sponsoring Translational and Epidemiological Research (MONSTER) Initiative. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Pesquisa Clínica e Translacional. Salvador, BA, Brasil. | en_US |
| Affilliation | Africa Health Research Institute. University of KwaZulu-Natal. Durban, South Africa / Department of Infectious Diseases. University of KwaZulu-Natal. Durban, South Africa / Department of Infection and Immunity. University College of London. London, United Kingdom. | en_US |
| Affilliation | Africa Health Research Institute. University of KwaZulu-Natal. Durban, South Africa / Department of Microbiology. University of Alabama at Birmingham. Birmingham, AL, United States / Centers for AIDS Research and Free Radical Biology. University of Alabama at Birmingham. Birmingham, AL, United States. | en_US |
| Subject | Tuberculosis | en_US |
| Subject | Bioenergetic metabolism | en_US |
| Subject | Lymphocytes | en_US |
| Subject | Monocytes | en_US |
| Subject | TB treatment | en_US |
| Subject | Cytokines | en_US |
| Subject | Seahorse XF96 | en_US |
| DeCS | Tuberculose | en_US |
| DeCS | Metabolismo | en_US |
| DeCS | Linfócitos | en_US |
| DeCS | Monócitos | en_US |
| DeCS | Citocinas | en_US |
