| Author | Vieira, Débora Ferreira Barreto | |
| Author | Miranda, Milene Dias | |
| Author | Silva, Marcos Alexandre Nunes da | |
| Author | Almeida, Andressa Santos de | |
| Author | Almeida, Ana Luisa Teixeira de | |
| Author | Bandeira, Derick Mendes | |
| Author | Ferreira, Vivian Neuza dos Santos | |
| Author | Rosa, Alice dos Santos | |
| Author | Dias, Wendell Girard | |
| Author | Archanjo, Bráulio Soares | |
| Author | Schatzmayr, Ortrud Monika Barth | |
| Access date | 2025-01-21T19:02:51Z | |
| Available date | 2025-01-21T19:02:51Z | |
| Document date | 2025 | |
| Citation | VIEIRA, Débora Ferreira Barreto et al. MPXV: update on morphological and morphogenesis aspects through transmission and scanning electron microscopies and 3D reconstruction. Journal of Medical Virology, v. 97, n. 1, p. 1-15, 18 Jan. 2025. | |
| ISSN | 0146-6615 | |
| URI | https://www.arca.fiocruz.br/handle/icict/68174 | |
| Description | Produção científica do Laboratório de Morfologia e Morfogênese Viral. | pt_BR |
| Sponsorship | The research was funded by Laboratório de Morfologia e Morfogênese Viral, Instituto Oswaldo Cruz (IOC), Fiocruz, FIOTEC (grant number IOC‐023‐FIO‐18‐2‐58), Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) (grant number: E‐27/2021/269465; E‐26/201.426/2022, E‐26/201.574/2021) to Débora Ferreira Barreto Vieira and Milene Dias Miranda. | |
| Language | eng | en_US |
| Publisher | Wiley | |
| Rights | open access | |
| Title | MPXV: update on morphological and morphogenesis aspects through transmission and scanning electron microscopies and 3D reconstruction | en_US |
| Type | Article | |
| DOI | 10.1002/jmv.70180 | |
| Abstract | An unprecedented global outbreak caused by the monkeypox virus (MPXV) prompted the World Health Organization to declare a public health emergency of international concern on July 23, 2022. Therapeutics and vaccines for MPXV are not widely available, necessitating further studies, particularly in drug repurposing area. To this end, the standardization of in vitro infection systems is essential. The most robust in vitro studies on poxviruses concern the Vaccinia virus, and there are significant gaps in understanding the replicative cycle of MPXV. Herein, we conducted ultrastructural studies using transmission and scanning electron microscopies and 3D reconstruction to describe and elucidate the step-by-step morphogenesis of MPXV. Vero cells, derived from the kidney lineage of Cercopithecus aethiops monkeys, were infected with a strain isolated from an oropharyngeal swab of a patient with suspected Mpox, collected during an observational cohort study conducted between June 12 and August 19, 2022, in Rio de Janeiro, Brazil. Infected Vero cells exhibited several morphological alterations, including cell lysis plaque formation, nuclei with altered chromatin profiles, thickening of the rough endoplasmic reticulum (RER), presence of myelin figures, disorganization of mitochondrial cristae, and the formation of a granular and fibrous matrix (viral factory) surrounded by mitochondria and RER cisternae in a perinuclear space. Viral entry into cells occurred via endocytosis MPXV particles were observed adhering to cytoskeletal filaments, and viral progeny extrusion occurred through exocytosis. This article presents novel data on the morphogenesis of MPXV that have not been previously documented in the literature. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Plataforma de Microscopia Eletrônica Rudolf Barth. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Instituto Nacional de Metrologia, Qualidade e Tecnologia. Laboratório de Materiais e Superfícies. Núcleo de Laboratório de Microscopia. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | |
| Subject | Mpox | en_US |
| Subject | MPXV | en_US |
| Subject | Scanning electron microscopy | en_US |
| Subject | Transmission electron microscopy | en_US |
| Subject | Tridimensional reconstruction | en_US |
| e-ISSN | 1096-9071 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
| xmlui.metadata.dc.subject.ods | 09 Indústria, inovação e infraestrutura | |
| xmlui.metadata.dc.subject.ods | 17 Parcerias e meios de implementação | |
