| Author | Campos, Gabriel Montenegro de | |
| Author | Costa, Thalita Cristina de Mello | |
| Author | Silveira, Roberta Maraninchi | |
| Author | Valença, Ian Nunes | |
| Author | Bezerra, Rafael Dos Santos | |
| Author | Darrigo Junior, Luiz Guilherme | |
| Author | Vieira, Ana Carolina de Jesus | |
| Author | Mesquita, Camila Campos | |
| Author | Laurindo, Patrícia da Silva | |
| Author | Cunha, Renato Guerino | |
| Author | Kashima, Simone | |
| Author | Covas, Dimas Tadeu | |
| Author | Simões, Belinda Pinto | |
| Author | Sampaio, Sandra Coccuzzo | |
| Author | Elias, Maria Carolina | |
| Author | Giovanetti, Marta | |
| Author | Slavov, Svetoslav Nanev | |
| Access date | 2025-01-23T19:29:17Z | |
| Available date | 2025-01-23T19:29:17Z | |
| Document date | 2024 | |
| Citation | DE CAMPOS, Gabriel Montenegro et al. Viral Metagenomics in Patients Who Underwent Allogeneic Hematopoietic Stem Cell Transplantation (HSCT): A Brazilian Experience. Microorganisms, v. 12, n. 12, 2557, 2024. | en_US |
| ISSN | 2076-2607 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/68221 | |
| Language | eng | en_US |
| Publisher | MDPI AG | en_US |
| Rights | open access | en_US |
| Title | Viral Metagenomics in Patients Who Underwent Allogeneic Hematopoietic Stem Cell Transplantation (HSCT): A Brazilian Experience. | en_US |
| Type | Article | en_US |
| DOI | 10.3390/microorganisms12122557 | |
| Abstract | Viral infections are one of the most important causes of morbidity and mortality among patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT). Immunosuppression may lead to the reactivation of latent viruses or the acquisition of new infections, resulting in severe clinical outcomes. The early detection of viral reactivations is crucial for effective patient management and post-transplant care. In this study, we employed next-generation metagenomics to assess changes in viral abundance and detect clinically significant viruses in allogeneic HSCT patients. A total of 20 patients from the Transplant Unit of the University Hospital of the Faculty of Medicine of Ribeirão Preto, University of São Paulo were included, with plasma samples collected at three time points: D + 0 (pre-transplantation), D + 30 (30 days post-transplantation), and D + 100 (~100 days post-transplantation). A higher presence of clinically relevant viruses, such as the cytomegalovirus (CMV), the Epstein-Barr virus (EBV) and adenoviruses, were predominantly detected at D + 30. The diversity of commensal viruses, primarily anelloviruses, increased gradually, with the highest abundance and variability detected at D + 100. Viruses with clinical importance for HSCT, including CMV, adenovirus and EBV, were confirmed and characterized at the molecular level, showing generally high cycle threshold values. Our findings demonstrate a rise in anellovirus abundance following allogeneic HSCT, with the highest levels observed at D + 100. Notably, D + 30 was identified as a critical time point for the reactivation of clinically significant viruses. This study underscores the potential of metagenomics in the identification of clinically relevant viruses and highlights the importance of monitoring latent viruses in immunocompromised populations, including allogeneic HSCT patients. | en_US |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Hemocentro de Ribeirão Preto. Ribeirão Preto, SP, Brasil. | en_US |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Imagem Médica, Hematologia e Oncologia. Ribeirão Preto, SP, Brasil. | en_US |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Hemocentro de Ribeirão Preto. Ribeirão Preto, SP, Brasil. | en_US |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Hemocentro de Ribeirão Preto. Ribeirão Preto, SP, Brasil. | en_US |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Hemocentro de Ribeirão Preto. Ribeirão Preto, SP, Brasil. | en_US |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Pediatria. Ribeirão Preto, SP, Brasil. | en_US |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Imagem Médica, Hematologia e Oncologia. Ribeirão Preto, SP, Brasil. | en_US |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Imagem Médica, Hematologia e Oncologia. Ribeirão Preto, SP, Brasil. | en_US |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Imagem Médica, Hematologia e Oncologia. Ribeirão Preto, SP, Brasil. | en_US |
| Affilliation | Oncolcínicas. Programa de Terapia Celular.,São Paulo, SP, Brasil. | en_US |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Hemocentro de Ribeirão Preto. Ribeirão Preto, SP, Brasil. | en_US |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Imagem Médica, Hematologia e Oncologia. Ribeirão Preto, SP, Brasil. | en_US |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Imagem Médica, Hematologia e Oncologia. Ribeirão Preto, SP, Brasil. | en_US |
| Affilliation | Instituto Butantan. Centro de Vigilância Viral e Avaliação Sorológica. São Paulo, SP, Brasil. | en_US |
| Affilliation | Instituto Butantan. Centro de Vigilância Viral e Avaliação Sorológica. São Paulo, SP, Brasil. | en_US |
| Affilliation | Department of Sciences and Technologies for Sustainable Development and One Health. Università Campus Bio-Medico di Roma. Rome, Italy. / Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | en_US |
| Affilliation | Instituto Butantan. Centro de Vigilância Viral e Avaliação Sorológica. São Paulo, SP, Brasil. | en_US |
| Subject | HSCT | en_US |
| Subject | TTV | en_US |
| Subject | commensal viruses | en_US |
| Subject | hematopoietic stem cell transplantation | en_US |
| Subject | infection | en_US |
| Subject | torque teno virus | en_US |
| Subject | viral metagenomics | en_US |
