| Author | Caldeira, Izabela Daniel Sardinha | |
| Author | Giovanini, Guilherme | |
| Author | Adorno, Lissandra Mara Ferreira | |
| Author | Santos, Débora Cristina Fernandes da Silva | |
| Author | Romero Ramos, Celso Raúl | |
| Author | Cruz Visalaya, Sergio Rafael | |
| Author | Pacheco Otalora, Luis Fernando | |
| Author | Siqueira, Flávia Ramos de | |
| Author | Dantas, Viviane Abreu Nunes Cerqueira | |
| Author | Belizário, José Ernesto | |
| Author | Garay Malpartida, Humberto Miguel | |
| Access date | 2025-01-28T14:29:23Z | |
| Available date | 2025-01-28T14:29:23Z | |
| Document date | 2024 | |
| Citation | CALDEIRA, Izabela Daniel Sardinha et al. Antiapoptotic and prometastatic roles of cytokine FAM3B in triple-negative breast cancer. Clinical Breast Cancer, v. 24, n. 7, p. e633-e644, Oct. 2024. | |
| ISSN | 1526-8209 | |
| URI | https://www.arca.fiocruz.br/handle/icict/68286 | |
| Description | Produção científica do Laboratório de Esquistossomose Experimental (vigente até jan/2023). | pt_BR |
| Sponsorship | This work was partially supported by the São Paulo State Research Foundation (FAPESP, Proc. 2007/04513-1) and the Brazilian National Council for Scientific and Technological Development (CNPq, Proc. 2011/486048). We are immensely grateful to the citizens of this country, whose taxes fund public universities and Brazilian science. We would also like to thank the entire technical team at the laboratory and the outsourced workers who help maintain the cleanliness of the scientific research spaces. Without the assistance of these individuals, it would have been even more challenging to endure the financial resource blockades during Bolsonaro's denialist government, which directly impacted the public university budget, R&D prospects, and the training of new scientists in our country. | |
| Language | eng | en_US |
| Publisher | Elsevier | |
| Rights | restricted access | |
| Title | Antiapoptotic and prometastatic roles of cytokine FAM3B in triple-negative breast cancer | en_US |
| Type | Article | |
| DOI | 10.1016/j.clbc.2024.06.008 | |
| Abstract | Background: Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer. FAM3B, a secreted protein, has been extensively studied in various types of tumors. However, its function in breast cancer remains poorly understood. Methods: We analyzed FAM3B expression data from breast cancer patients available at TCGA database and overall survival was analyzed by using the Kaplan-Meier plotter. MDA-MB-231 TNBC tumor cell line and hormone-responsive MCF-7 cell lines were transfected to overexpress FAM3B. We assessed cell death, tumorigenicity, and invasiveness in vitro through MTT analysis, flow cytometry assays, anchorage-independent tumor growth, and wound healing assays, respectively. We performed in vivo evaluation by tumor xenograft in nude mice. Results: In silico analysis revealed that FAM3B expression was lower in all breast tumors. However, TNBC patients with high FAM3B expression had a poor prognosis. FAM3B overexpression protected MDA-MB-231 cells from cell death, with increased expression of Bcl-2 and Bcl-xL, and reduced caspase-3 activity. MDA-MB-231 cells overexpressing FAM3B also exhibited increased tumorigenicity and migration rates in vitro, displaying increased tumor growth and reduced survival rates in xenotransplanted nude mice. This phenotype is accompanied by the upregulation of EMT-related genes Slug, Snail, TGFBR2, vimentin, N-cadherin, MMP-2, MMP-9, and MMP-14. However, these effects were not observed in the MCF-7 cells overexpressing FAM3B. Conclusion: FAM3B overexpression contributes to tumor growth, promotion of metastasis, and, consequently, leads to a poor prognosis in the most aggressive forms of breast cancer. Future clinical research is necessary to validate FAM3B as both a diagnostic and a therapeutic strategy for TNBC. | en_US |
| Affilliation | Universidade de São Paulo. Escola de Artes, Ciências e Humanidades. Conjuntos Multidisciplinares de Pesquisa. São Paulo, SP, Brasil. | |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. Instituto do Câncer do Estado de São Paulo. Centro de Investigação Translacional em Oncologia. São Paulo, SP, Brasil. | |
| Affilliation | Universidade de São Paulo. Escola de Artes, Ciências e Humanidades. Conjuntos Multidisciplinares de Pesquisa. São Paulo, SP, Brasil. | |
| Affilliation | Universidade de São Paulo. Escola de Artes, Ciências e Humanidades. Conjuntos Multidisciplinares de Pesquisa. São Paulo, SP, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidad Andina del Cusco. Cusco, Perú. | |
| Affilliation | Universidad Andina del Cusco. Cusco, Perú. | |
| Affilliation | Universidade de São Paulo. Escola de Artes, Ciências e Humanidades. Conjuntos Multidisciplinares de Pesquisa. São Paulo, SP, Brasil. | |
| Affilliation | Universidade de São Paulo. Escola de Artes, Ciências e Humanidades. Conjuntos Multidisciplinares de Pesquisa. São Paulo, SP, Brasil. | |
| Affilliation | Universidade de São Paulo. Escola de Artes, Ciências e Humanidades. Conjuntos Multidisciplinares de Pesquisa. São Paulo, SP, Brasil. | |
| Affilliation | Universidade de São Paulo. Escola de Artes, Ciências e Humanidades. Conjuntos Multidisciplinares de Pesquisa. São Paulo, SP, Brasil. | |
| Subject | Triple-negative breast cancer cytokines FAM3B EMT | en_US |
| Subject | FAM3 gene family | en_US |
| Subject | Cell death | en_US |
| Subject | Metastasis | en_US |
| e-ISSN | 1938-0666 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
