| Author | Silva, P. M. | |
| Author | Silva, J. N. O. | |
| Author | Silva, B. R. | |
| Author | Ferreira, G. R. S. | |
| Author | Gaião, W. D. C. | |
| Author | Recio, M. V. | |
| Author | Gonçalves, G. G. A. | |
| Author | Rodrigues, C. G. | |
| Author | Medeiros, P. L. | |
| Author | Brayner, F. A. | |
| Author | Alves, L. C. | |
| Author | Larsen, M. H. | |
| Author | Ingmer, H. | |
| Author | Napoleão, T. H. | |
| Author | Paiva, P. M. G. | |
| Access date | 2025-02-05T01:53:54Z | |
| Available date | 2025-02-05T01:53:54Z | |
| Document date | 2021 | |
| Citation | SILVA, P. M. et al. Antibacterial effects of the lectin from pomegranate sarcotesta (PgTeL) against Listeria monocytogenes. Journal of Applied Microbiology, v. 131, n. 2, p. 671-681, Aug. 2021. | en_US |
| ISSN | 1364-5072 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/68423 | |
| Language | eng | en_US |
| Publisher | Oxford | en_US |
| Rights | restricted access | |
| Title | Antibacterial effects of the lectin from pomegranate sarcotesta (PgTeL) against Listeria monocytogenes | en_US |
| Type | Article | |
| DOI | 10.1111/jam.14978 | |
| Abstract | Aims: To investigate the effects of the lectin from Punica granatum sarcotesta (PgTeL) on growth, viability, cell structure, biofilm formation and chitinase activity of Listeria monocytogenes. In addition, the effect of PgTeL on the adhesion and invasion of human cells (HeLa) was determined. Methods and results: PgTeL showed bacteriostatic and bactericidal effects on the strains L. monocytogenes N53-1 and EGD-e, causing morphometric alterations, cell aggregation, strong deformation and cell disruption. PgTeL inhibited biofilm formation by EGD-e and N53-1 and also interfered with the adhesion and invasion processes of EGD-e and N53-1 in HeLa cells. Finally, the chitinase activity of L. monocytogenes EGD-e was reduced in the presence of PgTeL, which can be involved in the inhibition of adhesion process. Conclusion: PgTeL is an antibacterial agent against L. monocytogenes, inhibiting growth and promoting cell death, as well as impairing biofilm formation and bacterial adhesion and invasion into human cells. Significance and impact of the study: The results stimulate future investigations on the potential of PgTeL for protection of contamination in food products. | en_US |
| Affilliation | Universidade Federal de Pernambuco. Centro de Biociências. Departamento de Bioquímica. Recife, PE, Brasil. | en_US |
| Affilliation | Universidade Federal de Pernambuco. Centro de Biociências. Departamento de Bioquímica. Recife, PE, Brasil. | en_US |
| Affilliation | Universidade Federal de Pernambuco. Centro de Biociências. Departamento de Bioquímica. Recife, PE, Brasil. | en_US |
| Affilliation | Universidade Federal de Pernambuco. Centro de Biociências. Departamento de Bioquímica. Recife, PE, Brasil. | en_US |
| Affilliation | Universidade Federal de Pernambuco. Centro de Biociências. Departamento de Biofísica e Radiobiologia. Recife, PE, Brasil. | en_US |
| Affilliation | University of Copenhagen. Department of Veterinary and Animal Sciences. Copenhagen, Denmark. | en_US |
| Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo Asami. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Recife, PE, Brasil. | en_US |
| Affilliation | Universidade Federal de Pernambuco. Centro de Biociências. Departamento de Biofísica e Radiobiologia. Recife, PE, Brasil. | en_US |
| Affilliation | Universidade Federal de Pernambuco. Centro de Biociências. Departamento de Histologia e Embriologia. Recife, PE, Brasil. | en_US |
| Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo Asami. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Recife, PE, Brasil. | en_US |
| Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo Asami. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Recife, PE, Brasil. | en_US |
| Affilliation | University of Copenhagen. Department of Veterinary and Animal Sciences. Copenhagen, Denmark. | en_US |
| Affilliation | University of Copenhagen. Department of Veterinary and Animal Sciences. Copenhagen, Denmark. | en_US |
| Affilliation | Universidade Federal de Pernambuco. Centro de Biociências. Departamento de Bioquímica. Recife, PE, Brasil. | en_US |
| Affilliation | Universidade Federal de Pernambuco. Centro de Biociências. Departamento de Bioquímica. Recife, PE, Brasil. | en_US |
| Subject | Biofilm | en_US |
| Subject | Food-borne infection | en_US |
| Subject | Lectin | en_US |
| Subject | Listeria monocytogenes | en_US |
| Subject | Pomegranate juice | en_US |
| e-ISSN | 1365-2672 | |
| Embargo date | 2030-12-31 | |
