| Author | Santana, Carla Andreia Abreu de | |
| Author | Silva, Gabriel Ferraz da | |
| Author | Santos, Rômulo Custodio dos | |
| Author | Maia, Luciana Conde Rodrigues | |
| Author | Dantas, Danillo Pereira | |
| Author | Archanjo, Bráulio Soares | |
| Author | Linden, Rafael | |
| Author | Coelho, Pedro Moreno Pimentel | |
| Author | Allodi, Silvana | |
| Access date | 2025-02-11T16:11:48Z | |
| Available date | 2025-02-11T16:11:48Z | |
| Document date | 2025 | |
| Citation | SANTANA, Carla Andreia Abreu de et al. Early ultrastructural damage in retina and optic nerve following intraocular pressure elevation. Vision Research, v. 227, n. 108544, p. 1-12, Feb. 2025. | |
| ISSN | 0042-6989 | |
| URI | https://www.arca.fiocruz.br/handle/icict/68506 | |
| Sponsorship | This study was supported by grants and fellowships from the Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and the Coordenação de Pessoal de Ensino Superior (CAPES/MEC), Brazil. | |
| Language | eng | en_US |
| Publisher | Elsevier | |
| Rights | restricted access | |
| Title | Early ultrastructural damage in retina and optic nerve following intraocular pressure elevation | en_US |
| Type | Article | |
| DOI | 10.1016/j.visres.2025.108544 | |
| Abstract | Elevated intraocular pressure (IOP) is a significant risk factor for glaucoma, causing structural and functional damage to the eye. Increased IOP compromises the metabolic and structural integrity of retinal ganglion cell (RGC) axons, leading to progressive degeneration and influencing the ocular immune response. This study investigated early cellular and molecular changes in the retina and optic nerve (ON) following ocular hypertension (OHT). A pigmented rat model was used, with OHT induced through low-temperature cauterization of the limbal vascular plexus. To assess the effects at early time points after OHT, transmission electron microscopy (TEM) was employed to analyze ultrastructural changes in the retina and ON, while immunofluorescence was used to evaluate cellular responses. Flow cytometry was used to examine alterations in immune-cell populations. Within 24 h post-OHT, ultrastructural changes were detected in the cytoplasm of RGCs, indicating early cellular alterations undetectable by conventional microscopy. These ultrastructural modifications progressed further at 48 and 72 h, despite the absence of overt RGC loss or disruptions in retinal layer integrity. Changes in the axons and nodes of Ranvier were evident within the first 24 h after ocular hypertension, becoming more pronounced by 72 h. These findings offer novel insights into the early pathogenesis of glaucoma, highlighting critical early impacts that could guide the development of new therapeutic strategies to prevent irreversible RGC loss. | en_US |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Microscopia Eletrônica Rudolf Barth. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Ministério do Desenvolvimento, Indústria, Comércio e Serviços. Instituto Nacional de Metrologia, Qualidade e Tecnologia. Divisão de Metrologia de Materiais. Duque de Caxias, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | |
| Subject | Glaucoma | en_US |
| Subject | Intraocular pressure (IOP) | en_US |
| Subject | Retinal ganglion cell | en_US |
| Subject | Optic nerve | en_US |
| e-ISSN | 1878-5646 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
| xmlui.metadata.dc.subject.ods | 09 Indústria, inovação e infraestrutura | |
