| Author | Couto, Flávia Fernanda Bubula | |
| Author | Coelho, Paulo Marcos Zach | |
| Author | Pereira, Neusa Araújo | |
| Author | Kusel, John Robert | |
| Author | Katz, Naftale | |
| Author | Mattos, Ana Carolina Alves de | |
| Access date | 2013-09-10T13:28:38Z | |
| Available date | 2013-09-10T13:28:38Z | |
| Document date | 2010 | |
| Citation | COUTO, Flávia Fernanda Bubula et al. Use of fluorescent probes as a useful tool to identify resistant Schistosoma mansoni isolates to praziquantel. Parasitology, v. 137, n. 12, p. 1791-1797, Oct. 2010. | pt_BR |
| ISSN | 0031-1820 | |
| URI | https://www.arca.fiocruz.br/handle/icict/6865 | |
| Language | eng | pt_BR |
| Publisher | Cambridge Journals | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Use of fluorescent probes as a useful tool to identify resistant Schistosoma mansoni isolates to praziquantel | pt_BR |
| Type | Article | |
| DOI | 10.1017/S003118201000065X | |
| Abstract | The use of chemotherapy on a mass scale in endemic areas may lead to the appearance of resistant isolates through the mechanism of selective drug pressure. Studies have demonstrated that praziquantel (PZQ) is able to inhibit the excretory activity and to cause tegumental damage in Schistosoma mansoniadult worms. The use of the probe resorufin to evaluate excretory activity, as well as the probe Hoechst 33258 to detect tegumental damage in adult worms, may represent a method to identify resistant (or less susceptible) isolates. The purpose of the present work was to compare the changes caused by PZQ in the function of the excretory system and in the integrity of the tegument of adult worms from the LE isolate (susceptible to PZQ) and the LE-PZQ isolate (less susceptible to PZQ). Worms from the isolateLE-PZQshowed less severe tegumental lesions, in bothin vitro andin vivo experiments, detected by labelling with Hoechst 33258 and continued to have a functional excretory system as shown by labelling with resorufinin vitro. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG,Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | University of Glasgow. Division of Infection and Immunity. Scotland, UK. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | drug resistance, praziquantel | pt_BR |
| Subject | fluorescent probes | pt_BR |
