| Author | Miranda, Vanessa do Socorro Cabral | |
| Author | Falcão, Luiz Fabio Magno | |
| Author | Fuzii, Hellen Thais | |
| Author | Carvalho, Marcos Luiz Gaia | |
| Author | Lopes, Jeferson da Costa | |
| Author | Martins Filho, Arnaldo Jorge | |
| Author | Cruz, Ana Cecilia Ribeiro | |
| Author | Azevedo, Raimunda do Socorro da Silva | |
| Author | Sousa, Jorge Rodrigues de | |
| Author | Wakimoto, Mayumi Duarte | |
| Author | Vasconcelos, Pedro Fernando da Costa | |
| Author | Quaresma, Juarez Antônio Simões | |
| Access date | 2025-02-25T01:34:49Z | |
| Available date | 2025-02-25T01:34:49Z | |
| Document date | 2024 | |
| Citation | MIRANDA, Vanessa do Socorro Cabral et al. Analysis of MLKL, RIP1 and RIP3 Immunostaining Markers in Human Liver Tissue from Fatal Yellow Fever Cases: Insights into Necroptosis. Viruses, v. 17, n. 3, p. 1-10, Dec. 2024. | en_US |
| ISSN | 1999-4915 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/68706 | |
| Sponsorship | This study was supported by the Brazilian Council for the Scientific and Technologic Development Agency (CNPq) for financial support to PFCV (grants 457664/2013–4 and 303999/2016–0). | en_US |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | en_US |
| Title | Analysis of MLKL, RIP1 and RIP3 Immunostaining Markers in Human Liver Tissue from Fatal Yellow Fever Cases: Insights into Necroptosis | en_US |
| Type | Article | en_US |
| DOI | 10.3390/v17010003 | |
| Abstract | Necroptosis is a regulated form of cell death implicated in several pathological conditions, including viral infections. In this study, we investigated the expression and correlation of necroptosis markers MLKL, RIP1 and RIP3 in human liver tissue from fatal cases of yellow fever (YF) using immunohistochemistry (IHC). The liver samples were obtained from 21 YF-positive individuals and five flavivirus-negative controls with preserved liver parenchymal architecture. The cases underwent histopathological analysis, followed by tissue immunostaining with the immunohistochemical method of streptavidin-biotin peroxidase. Using the in situ method, we evaluated the centrilobular zone (Z3), midzonal zone (Z2), periportal zone and portal tract (PT) of human liver parenchyma with markers for necroptosis, RIPK1, RIPK3 and MLKL. A quantitative analysis revealed a significantly higher expression of MLKL, RIP1 and RIP3 in the liver parenchyma of YF cases compared to controls in different zones (Z3, Z2, Z1) and portal tracts (PTs) of the liver, especially in zone 2. Immunostaining confirmed the localization of MLKL, RIP1 and RIP3 in hepatocytes and inflammatory infiltrates, highlighting their involvement in the pathogenesis of YF. A Pearson correlation analysis demonstrated significant correlations among necroptosis markers, which indicates their coordinated regulation during YF-induced liver injury. | en_US |
| Affilliation | Ministry of Health. Evandro Chagas Institute. Departmento of Pathology. Ananindeua, PA, Brazil. | en_US |
| Affilliation | State University of Para. Departmento of Pathology. Belem, PA, Brazil. | en_US |
| Affilliation | Federal University of Para. Tropical Medicine Center. Belem, PA, Brazil. | en_US |
| Affilliation | Ministry of Health. Evandro Chagas Institute. Departmento of Pathology. Ananindeua, PA, Brazil. | en_US |
| Affilliation | Ministry of Health. Evandro Chagas Institute. Departmento of Pathology. Ananindeua, PA, Brazil. | en_US |
| Affilliation | Ministry of Health. Evandro Chagas Institute. Departmento of Pathology. Ananindeua, PA, Brazil. | en_US |
| Affilliation | Ministry of Health. Evandro Chagas Institute. Departmento of Pathology. Ananindeua, PA, Brazil. | en_US |
| Affilliation | Ministry of Health. Evandro Chagas Institute. Departmento of Pathology. Ananindeua, PA, Brazil. | en_US |
| Affilliation | Ministry of Health. Evandro Chagas Institute. Departmento of Pathology. Ananindeua, PA, Brazil / State University of Para. Departmento of Pathology. Belem, PA, Brazil / Federal University of Para. Tropical Medicine Center. Belem, PA, Brazil. | en_US |
| Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Ministry of Health. Evandro Chagas Institute. Departmento of Pathology. Ananindeua, PA, Brazil / State University of Para. Departmento of Pathology. Belem, PA, Brazil. | en_US |
| Affilliation | Ministry of Health. Evandro Chagas Institute. Departmento of Pathology. Ananindeua, PA, Brazil / State University of Para. Departmento of Pathology. Belem, PA, Brazil / Federal University of Para. Tropical Medicine Center. Belem, PA, Brazil / Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Rio de Janeiro, RJ, Brazil / Sao Paulo University. School of Medicine. Department of Infectious Disease. Sao Paulo, SP, Brazil. | en_US |
| Subject | Yellow fever | en_US |
| Subject | Necroptosis | en_US |
| Subject | Liver parenchyma | en_US |
| e-ISSN | 1999-4915 | |
