| Author | Pedro, Giovana | |
| Author | Brasileiro, Felipe César da Silva | |
| Author | Macedo, Jamile Mariano | |
| Author | Soares, Andreimar Martins | |
| Author | Mafra, Gabriel Caporale | |
| Author | Alves, Carlos Eduardo Fonseca | |
| Author | Laufer-Amorim, Renée | |
| Access date | 2025-03-06T14:47:49Z | |
| Available date | 2025-03-06T14:47:49Z | |
| Document date | 2024 | |
| Citation | PEDRO, Giovana et al. Cytotoxic effects of crotoxin from Crotalus durissus terrificus snake in canine mammary tumor cell lines. J Venom Anim Toxins incl Trop Dis, v. 30, n. 30:e20230062, p. 1-10, 18 Mar. 2024. | en_US |
| ISSN | 1678-9199 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/68797 | |
| Sponsorship | FAPESP | en_US |
| Language | eng | en_US |
| Publisher | Centro de Estudos de Venenos e Animais Peçonhentos CEVAP/UNESP | en_US |
| Rights | open access | en_US |
| Title | Cytotoxic effects of crotoxin from Crotalus durissus terrificus snake in canine mammary tumor cell lines | en_US |
| Type | Article | en_US |
| DOI | 10.1590/1678-9199-JVATITD-2023-0062 | |
| Abstract | Background: Mammary gland tumors are the most prevalent neoplasm in intact female dogs, and they are good natural models to study comparative oncology. Most canine mammary malignancies, as in women, are commonly refractory to conventional therapies and demand continuous new therapeutic approaches. Crotalus durissus terrificus, also called rattlesnake, has more than 60 different proteins in its venom with multiple pharmaceutical uses, such as antitumor, antiviral, and antimicrobial action. Crotoxin, a potent β-neurotoxin formed by the junction of two subunits, a basic subunit (CBPLA 2) and an acidic subunit (crotapotin), has already been reported to have anticancer properties in different types of cancers. Methods: In this work, we describe the cytotoxic potential of crotoxin and its subunits compared to doxorubicin (drug of choice) in two canine mammary carcinoma cell lines. Results: Crotoxin, CB-PLA2, crotalic venom,
and doxorubicin decreased cell viability and the ability to migrate in a dose-dependent manner, and crotapotin did not present an antitumoral effect. For all compounds, the predominant cell death mechanism was apoptosis. In addition, crotoxin did not show toxicity in normal canine mammary gland cells. Conclusion: Therefore, this work showed that crotoxin and CB-PLA2 had cytotoxic activity, migration inhibition, and pro-apoptotic potential in canine mammary gland carcinoma cell lines, making their possible use in cancer research. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de Biotecnologia de Proteínas e Compostos Bioativos. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Porto Velho, RO, Brasil / Faculdade São Lucas. Porto Velho, RO, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de Biotecnologia de Proteínas e Compostos Bioativos. Porto Velho, RO, Brasil / Fundação Universidade Federal de Rondônia. Porto Velho, RO, Brasil / Faculdade São Lucas. Porto Velho, RO, Brasil / Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de Biotecnologia de Proteínas e Compostos Bioativos. Rede de Excelência em Pesquisa e Conhecimento da Amazônia Ocidental. Porto Velho, RO, Brasil / Fundação Oswaldo Cruz. Fiocruz Rondônia. Instituto Nacional de Epidemiologia na Amazônia Ocidental. Porto Velho, RO, Brasil. | en_US |
| Affilliation | São Paulo State University. School of Veterinary Medicine and Animal Science. Botucatu, SP, Brasil. | en_US |
| Affilliation | São Paulo State University. School of Veterinary Medicine and Animal Science. Botucatu, SP, Brasil. | en_US |
| Affilliation | São Paulo State University. School of Veterinary Medicine and Animal Science. Botucatu, SP, Brasil. | en_US |
| Affilliation | São Paulo State University. School of Veterinary Medicine and Animal Science. Botucatu, SP, Brasil. | en_US |
| Affilliation | Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia. Botucatu, SP, Brasil / Fundação Oswaldo Cruz. Fiocruz Rondônia. Laboratório de Biotecnologia de Proteínas e Compostos Bioativos. Rede de Excelência em Pesquisa e Conhecimento da Amazônia Ocidental. Porto Velho, RO, Brasil / Fundação Oswaldo Cruz. Fiocruz Rondônia. Instituto Nacional de Epidemiologia na Amazônia Ocidental. Porto Velho, RO, Brasil. | en_US |
| Subject | Mammary tumor | en_US |
| Subject | Comparative oncology | en_US |
| Subject | Crotalus durissus terrificus venom | en_US |
| Subject | Biological compounds | en_US |
| DeCS | Breast Neoplasms | en_US |
| DeCS | Medical Oncology | en_US |
| DeCS | Crotalus cascavella | en_US |
