| Author | Mendes Júnior, Artur Augusto Velho | |
| Author | Figueiredo, Fabiano Borges | |
| Author | Ferreira, Luiz Cláudio | |
| Author | Keidel, Lucas | |
| Author | Ornellas, Renato Orsini | |
| Author | Almeida, Adilson Benedito | |
| Author | Santos, Fernanda Nunes | |
| Author | Miranda, Luciana de Freitas Campos | |
| Author | Marcelino, Andreza Pain | |
| Author | Pereira, Sandro Antonio | |
| Author | Menezes, Rodrigo Caldas | |
| Access date | 2025-03-10T23:04:58Z | |
| Available date | 2025-03-10T23:04:58Z | |
| Document date | 2025 | |
| Citation | MENDES JÚNIOR, Artur Augusto Velho et al. Performance of Culture Using a Semi-Automatic Needle as a Novel Tool for Collecting Lymph Node Samples for the Diagnosis of Canine Visceral Leishmaniasis. Animals, v. 15, n. 1, p. 1-9, Jan. 2025. | en_US |
| ISSN | 2076-2615 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/68881 | |
| Sponsorship | This research was funded by Coordenação de Aperfeicoamento de Pessoal de Nível Superior (CAPES), Brazil (Finance Code 001), and by Fundacão Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Brazil (Grants CNE-E-26/201.032/2021 and E-26/200.791/2020). R.C.M., F.B.F., and S.A.P. are recipients of productivity fellowships from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil (Grants 305956/2021-3, 316975/2023-0 and 307067/2023-8). | en_US |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | en_US |
| Title | Performance of Culture Using a Semi-Automatic Needle as a Novel Tool for Collecting Lymph Node Samples for the Diagnosis of Canine Visceral Leishmaniasis | en_US |
| Type | Article | en_US |
| DOI | 10.3390/ani15010107 | |
| Abstract | Zoonotic visceral leishmaniasis is caused by Leishmania (Leishmania) infantum and dogs are the main domestic reservoir. This study compared the performance of parasitological tests using semi-automatic needle puncture (SANP) for collecting popliteal lymph node samples with samples collected from the same lymph node by fine needle aspiration puncture (FNAP) and by necropsy for the diagnosis of canine visceral leishmaniasis (CVL). Popliteal lymph node samples were collected from 30 CVL-seropositive dogs from an endemic region in Brazil. After clinical examination and euthanasia, samples were collected from the same lymph node by SANP, FNAP, and necropsy. The reference tests were culture, immunohistochemistry, and histopathology. Positivity for Leishmania spp. was 70% for immunohistochemistry and 33.3% for histopathology. Culture positivity using the different sampling techniques was 77% for necropsy (87% in the first week), 73% for FNAP (82% in the first week), and 63% for SANP (95% in the first week). The combination of SANP and culture proved to be an alternative for the diagnosis of Leishmania spp. in the lymph node samples of dogs because of its high positivity rate and because it is more practical and faster and has a shorter time to positivity by culture when compared to FNAP and necropsy sampling. | en_US |
| Affilliation | Oswaldo Cruz Foundation. Carlos Chagas Institute. Curitiba, PR, Brazil. | en_US |
| Affilliation | Oswaldo Cruz Foundation. Carlos Chagas Institute. Curitiba, PR, Brazil. | en_US |
| Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Laboratory of Clinical Research and Surveillance in Leishmaniasis. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Laboratory of Clinical Research and Surveillance in Leishmaniasis. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Laboratory of Clinical Research and Surveillance in Leishmaniasis. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals. Rio de Janeiro, RJ, Brazil. | en_US |
| Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Laboratory of Clinical Research on Dermatozoonoses in Domestic Animals. Rio de Janeiro, RJ, Brazil. | en_US |
| Subject | Canine visceral leishmaniasis | en_US |
| Subject | Semi-automatic needle | en_US |
| Subject | Parasitological diagnosis | en_US |
| Subject | Popliteal lymph node | en_US |
| Subject | Dogs | en_US |
| e-ISSN | 2076-2615 | |
