| Author | Freire, Luciana de Souza | |
| Author | Gandarilla, Ariamna María Dip | |
| Author | Barcelay, Yonny Romaguera | |
| Author | Ruzo, Camila Macena | |
| Author | Salgado, Barbara Batista | |
| Author | Tavares, Ana P. M. | |
| Author | Nobre, Francisco Xavier | |
| Author | Souza Neto, Julio Nino de | |
| Author | Astolfi Filho, Spartaco | |
| Author | Țălu, Ștefan | |
| Author | Lalwani, Pritesh | |
| Author | Patra, Niranjan | |
| Author | Brito, Walter Ricardo | |
| Access date | 2025-03-16T18:08:57Z | |
| Available date | 2025-03-16T18:08:57Z | |
| Document date | 2025 | |
| Citation | FREIRE, Luciana de Souza et al. Carboxylated Graphene: an Innovative Approach to Enhanced IgA-SARS-CoV-2 Electrochemical Biosensing. Biosensors Basel, v. 15, p. 1-15, Jan. 2025. | en_US |
| ISSN | 2079-6374 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/69030 | |
| Description | This study was conducted in accordance with the Declaration of Helsinki and approved by the Research Ethics Committee of the Federal University of Amazonas (CAAE:34906920.4.0000.5020-15/10/2020) for studies involving humans. | en_US |
| Sponsorship | Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | en_US |
| Language | eng | en_US |
| Rights | open access | en_US |
| Title | Carboxylated Graphene: An Innovative Approach to Enhanced IgA-SARS-CoV-2 Electrochemical Biosensing | en_US |
| Type | Article | en_US |
| DOI | 10.3390/bios15010034 | |
| Abstract | Biosensors harness biological materials as receptors linked to transducers, enabling the capture and transformation of primary biorecognition signals into measurable outputs. This study presents a novel carboxylation method for synthesizing carboxylated graphene (CG) under acidic conditions, enhancing biosensing capabilities. The characterization of the CG was performed using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), Raman spectroscopy, thermogravimetric analysis (TGA), and X-ray diffraction (XRD).We modified screen-printed carbon electrodes (SPCEs) with CG to immobilize the SARS-CoV-2 N-protein, facilitating targeted detection of IgA antibodies
(IgA-SARS-CoV-2). The analytical performance was assessed via electrochemical techniques such as cyclic voltammetry and electrochemical impedance spectroscopy, confirming CG synthesis effectiveness and biosensor functionality. The developed biosensor efficiently detects IgA-SARS-CoV-2 across a dilution range of 1:1000 to 1:200 v/v in a phosphate-buffered saline (PBS) solution, with a limit of detection calculated at 1:1601 v/v. This device shows considerable potential because of its fast response time, miniaturized design facilitated by SPCEs, reduced sample volume requirements, high sensitivity and specificity, low detection limits, and signal enhancement achieved through nanomaterial integration. | en_US |
| Affilliation | Universidade Federal do Amazonas. Manaus, AM, Brasil. | en_US |
| Affilliation | Universidade Federal do Amazonas. Manaus, AM, Brasil. | en_US |
| Affilliation | Universidade Federal do Amazonas. Manaus, AM, Brasil. | en_US |
| Affilliation | Universidade Federal do Amazonas. Manaus, AM, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Leônidas & Maria Deane. Manaus, AM, Brasil. | en_US |
| Affilliation | Centro de Nanotecnologia e Materiais Técnicos, Funcionais e Inteligentes. Lisboa, Portugal. | en_US |
| Affilliation | Instituto Federal do Amazonas. Manaus, AM, Brasil. | en_US |
| Affilliation | Universidade Federal do Amazonas. Manaus, AM, Brasil. | en_US |
| Affilliation | Universidade Federal do Amazonas. Manaus, AM, Brasil. | en_US |
| Affilliation | The Technical University of Cluj-Napoca. Romania | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Leônidas & Maria Deane. Manaus, AM, Brasil. | en_US |
| Affilliation | Koneru Lakshmaiah Education Foundation. India. | en_US |
| Affilliation | Universidade Federal do Amazonas. Manaus, AM, Brasil. | en_US |
| Subject | COVID-19 | en_US |
| Subject | IgA-SARS-CoV-2 | en_US |
| Subject | Carboxylated graphene | en_US |
| Subject | Electrochemical biosensors | |
| Subject | Screen-printed carbon electrodes | |
| DeCS | COVID-19 | en_US |
