| Author | Lima, Graciela Kunrath | |
| Author | Zolini, Guilherme Pimenta de Pádua | |
| Author | Mansur, Daniel Santos | |
| Author | Lima, Braulio Henrique Freire | |
| Author | Wischhoff, Uschi | |
| Author | Astigarraga, Ruiz Gerhardt | |
| Author | Dias, Marcela França | |
| Author | Silva, Mariana das Graças Almeida | |
| Author | Béla, Samantha Ribeiro | |
| Author | Antonelli, Lis Ribeiro do Valle | |
| Author | Arantes, Rosa Maria Esteves | |
| Author | Gazzinelli, Ricardo Tostes | |
| Author | Bafica, Andre Luiz Barbosa | |
| Author | Kroon, Erna Geessien | |
| Author | Campos, Marco Antônio da Silva | |
| Access date | 2013-09-17T14:25:19Z | |
| Available date | 2013-09-17T14:25:19Z | |
| Document date | 2010 | |
| Citation | LIMA, Graciela Kunrath e al. Toll-like receptor (TLR) 2 and TLR9 expressed in trigeminal ganglia are critical to viral control during herpes simplex virus 1 infection. The American Journal of Pathology, v. 177, n. 5, p. 2433-2445, Nov. 2010. | pt_BR |
| ISSN | 0002-9440 | |
| URI | https://www.arca.fiocruz.br/handle/icict/6921 | |
| Language | eng | pt_BR |
| Publisher | American Society for Investigative Pathology | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Toll-like receptor (TLR) 2 and TLR9 expressed in trigeminal ganglia are critical to viral control during herpes simplex virus 1 infection | pt_BR |
| Type | Article | |
| DOI | 10.2353/ajpath.2010.100121 | |
| Abstract | Herpes simplex virus 1 (HSV-1) is a neurotropic DNA virus that is responsible for several clinical manifestations in humans, including encephalitis. HSV-1 triggers toll-like receptors (TLRs), which elicit cytokine production. Viral multiplication and cytokine expression in C57BL/6 wild-type (WT) mice infected with HSV-1 were evaluated. Virus was found in the trigeminal ganglia (TG), but not in the brains of animals without signs of encephalitis, between 2 and 6 days postinfection (d.p.i.). Cytokine expression in the TG peaked at 5 d.p.i. TLR9-/- and TLR2/9-/- mice were more susceptible to the virus, with 60% and 100% mortality, respectively, as opposed to 10% in the WT and TLR2-/- mice. Increased levels of both CXCL10/IP-10 and CCL2/MCP-1, as well as reduced levels of interferon-γ and interleukin 1-β transcripts, measured in both the TG and brains at 5 d.p.i., and the presence of virus in the brain were correlated with total mortality in TLR2/9-/- mice. Cytokine alterations in TLR2/9-/- mice coincided with histopathological changes in their brains, which did not occur in WT and TLR2-/- mice and occurred only slightly in TLR9-/- mouse brain. Increased cellularity, macrophages, CD8 T cells producing interferon-γ, and expression levels of TLR2 and TLR9 were detected in the TG of WT-infected mice. We hypothesize that HSV-1 infection is controlled by TLR-dependent immune responses in the TG, which prevent HSV-1 encephalitis. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil / University of Massachusetts Medical School. Worcester, MA, USA. | pt_BR |
| Affilliation | Universidade Federal de Santa Catarina. Laboratorio de Imunologia e Doenças Infecciosas. Florianópolis, SC, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil. | pt_BR |
| Subject | Chemokines/immunology | pt_BR |
| Subject | Herpesvirus 1 | pt_BR |
| Subject | Human/immunology | pt_BR |
| Subject | Interleukin-12 Subunit p40/immunology | pt_BR |
| Subject | Tumor Necrosis Factor-alpha/immunology | pt_BR |
